CULTURE MEDIUM FOR FERMENTING TOLYPOCLADIUM INFLATUM TO PRODUCE LIPASE, AND CULTURE METHOD THEREBY

摘要

A culture medium for fermenting Tolypocladium inflatum to produce a lipase, and a culture method thereby. The enzyme production fermentation culture medium comprises: 2% (w/v) of emulsified canola oil, 2% (w/v) of D-fructose, 4% (w/v) of soy peptone, 0.1% (w/v) of FeSO ₄•7H ₂O, wherein the pH is 5 or the pH is 7, and the balance of water. Compared with the prior art, the culture medium for fermenting Tolypocladium inflatum to produce a lipase improves the enzyme activity from 806.07 U/mL of the basic fermentation medium to 4735.16 U/mL.