CULTURE MEDIUM FOR PRODUCING LIPASE BY FERMENTING TOLYPOCLADIUM GEODES AND CULTURE METHOD THEREFOR

摘要

Provided are a culture medium for producing lipase by fermenting Tolypocladium geodes and a culture method therefor. The culture medium consists of: 2％ (w/v) of emulsified canola oil, 2％ (w/v) of alpha-lactose, 4％ (w/v) of tryptone, 0.1％ (w/v) of MnSO ₄·H ₂O, and 0.1％ (w/v) of NH ₄NO ₃, pH = 5-10, and the balance of water; the enzyme activity of the lipase is improved to 6011.69 U/mL from 102.69 U/mL of a basic fermentation culture medium, and large-scale production thereof can be achieved.