METHOD FOR DETECTING MULTIPLE TARGETS BASED ON SINGLE DETECTION PROBE USING TAG SEQUENCE SNP

摘要

The present invention relates to a method for detecting multiple targets based on a single detection probe and, more specifically, to a method for detecting multiple targets, in which individual targets are amplified with respective primers containing SNP-bearing tag sequences and then hybridized with a single detection probes designed to bind to each of the tag sequences and to differentiate the melting points among the hybrids and the melting curves are analyzed. The multiple-target detection method according to the present invention can detect multiple targets by using a single probe. Thus, the multiple-target detection method is useful because it exhibits low false positives upon detecting multiple targets and can detect multiple targets at high sensitivity and speed.