Method for Detecting Analytes Based on Evanescent Illumination and Scatter-Based Detection of Nanoparticle Probe Complexes.

摘要

The invention provides methods of detecting one or more specific binding analytes, such as nucleic acids and proteins, in the presence of a neutral or anionic polysaccharide, through light scattering techniques, where a change in light scattering caused by the formation of nanoparticle label complexes within the penetration depth of the evanescent wave of a wave guide signals the presence of the analyte.