METHOD OF DETECTING MULTIPLE TARGETS BASED ON A SINGLE DETECTOR PROBE USING A MARKING SEQUENCE SNP

摘要

The present invention relates to a method for the detection of multiple targets on the basis of a single detection probe and, in particular, to a method for the detection of multiple targets by amplifying each target with primers containing an SNP-containing marker sequence, hybridizing the amplification products with a single detection probe which is able to bind to the marker sequences and is designed so that the melting temperatures are different from each other, and analysis of the melting curves. A method for detecting multiple targets according to the present invention enables multiple targets to be detected using a single probe and is therefore useful for multiple target detection because false positive results are reduced and multiple targets can be detected with high sensitivity and at a rapid rate .