PCR Nucleic acid complex pair competitive structure PCR kit using the same

摘要

According to an embodiment of the present application, in a nucleic acid complex pair, a first nucleic acid complex including a first determination unit, a first connection unit, a first pairing unit, and a first detection unit; And a second nucleic acid complex comprising a second determination unit, a second connection unit, a second pairing unit, and a second detection unit, wherein the first connection unit is located between the first determination unit and the first pairing unit, and the The first pairing unit is located between the first detection unit and the first connection unit, the first determining unit is a DNA strand containing a forward primer for a target DNA, and the first pairing unit is a DNA strand shorter than the first determining unit. , The second connection part is located between the second determination part and the second pairing part, the second pairing part is located between the second detection part and the second connection part, and the second determination part reverses the target DNA. A DNA strand containing a primer, the second pairing portion is a DNA strand shorter than the second determining portion, the first pairing portion has a base sequence complementary to the second pairing portion, and the first detection portion is the second A nucleic acid complex pair having characteristics corresponding to the detection unit may be provided.