PROCÉDÉ DE DÉTECTION PRÉCOCE DU CANCER DU CÔLON ET/OU DE CELLULES PRÉCURSEURS DU CANCER DU CÔLON, ET DE SURVEILLANCE DE LA RÉCURRENCE DU CANCER DU CÔLON

摘要

The invention provides a kit for detecting the presence or absence of mutations in the selected regions of the target genes associated with colorectal cancer, comprising XNA clamps and primers; wherein the XNA clamps are capable of hybridizing with the selected regions having wild-type sequences in the target genes, and the primers are capable of amplifying the selected regions containing each of the mutations in the target genes. The invention also discloses a method of detecting a mutant gene associated with colorectal cancer, comprising: providing a sample containing DNA and a xeno nucleic acid clamp capable of hybridizing to a wild-type gene; and detecting a mutant of the gene in the sample with a xeno nucleic acid probe capable of hybridizing to the mutant gene.