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基因突变序列及其在鉴定膀胱癌干细胞中的应用

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摘要

本发明公开了一种基因突变序列，与表1中的任意一个突变所在的基因序列相比，该基因突变序列包括存在该任意一个突变的基因序列。本发明还公开一种包含能够检测出所列基因突变序列的试剂的试剂盒和一种鉴定膀胱癌干细胞的方法。利用本发明的基因突变序列能够将膀胱癌干细胞从膀胱癌细胞和膀胱组织细胞中准确的区分出来。

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公开/公告号CN106636116B

专利类型发明专利
公开/公告日2020-09-15

原文格式PDF
申请/专利权人深圳市第二人民医院;
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申请/专利号CN201510464078.3
发明设计人吴松;蔡志明;杨昭;
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申请日2015-07-31
分类号C12N15/12(20060101);C12Q1/6886(20180101);
代理机构11201 北京清亦华知识产权代理事务所(普通合伙);
代理人李志东
地址 518035 广东省深圳市福田区笋岗西路3002号
入库时间 2022-08-23 11:13:40

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1. 基因突变序列及其在鉴定膀胱癌干细胞中的应用 [P] . 中国专利： CN106636116B . 2020.09.15
2. STAG2基因突变序列、其检测方法以及STAG2基因突变在检测膀胱癌中的应用 [P] . 中国专利： CN104059966A . 2014-09-24
3. Nucleotide sequences of the mutant and non-mutant fragments of loci A and C of alleles of double low (00) fad3 form desaturase gene and low-linolenic mutant (LLMut) of oilseed rape plants (Brassica napus L. var. Oleifera), allospecific SNP markers specific for loci A and C of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLMut) of winter oilseed rape plants, nucleotide sequences of primer pairs for the amplification of loci A and C of fad3 form desaturase gene of winter oilseed rape plants, method for amplification of loci A and C of fad3 desaturase gene of winter oilseed rape plants, nucleotide sequences of the primers for the identification of mutant and non-mutant alleles of fad3 forms desaturase gene - double low (00) and low-linolenic mutant (LLMut) of oilseed rape in locus A and locus C in the microsequencing reaction, method for the identification of mutant and non-mutant alleles of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLM [P] . PL211126B1 . 2012-04-30

机译：双低（00）fad3等位基因的等位基因双低等位基因（A）的位点A和C的突变和非突变片段的核苷酸序列形成去饱和酶基因和油菜植物（甘蓝型油菜）的低亚麻酸突变体（LLMut），是同种异体对fad3基因座A和C特异的SNP标记形成去饱和酶基因-冬季油菜植物的双低（00）和低亚麻酸突变体（LLMut），用于扩增fad3基因去饱和酶A和C的引物对的核苷酸序列冬季油菜植物基因，fad3去饱和酶基因的基因座A和C的扩增方法，用于鉴定fad3形式脱氢酶基因的突变和非突变等位基因的引物的核苷酸序列-双低（00 ）和基因座A和基因座C中油菜的低亚麻酸突变体（LLMut）的微测序反应，fad3形式脱氢酶基因突变和非突变等位基因的鉴定方法-双低（00）和低亚麻酸突变体（法学硕士
4. Process for identifying translated expression proteins from a nucleotide sequence, purified recombinant protein, nucleotide sequence, screening processes for identifying expression clones in a library, for screening a library of nucleotide sequences, for identifying antigenic gene sequences for a sample of nucleotide sequences and to identify a therapeutic antigenic gene sequence, recombinant vector, host cell, amino acid sequence, isolated and purified polypeptide, antibody, and process for treating an infection. [P] . BR9915066A . 2002-01-15

机译：从核苷酸序列，纯化的重组蛋白，核苷酸序列鉴定翻译的表达蛋白的方法，在文库中鉴定表达克隆，筛选核苷酸序列的文库，鉴定核苷酸序列样品的抗原基因序列并鉴定的筛选方法治疗性抗原基因序列，重组载体，宿主细胞，氨基酸序列，分离和纯化的多肽，抗体以及治疗感染的方法。
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途