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一种苹果酸酶基因RKME1及其重组表达载体

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摘要

本发明公开了一种从红冬孢酵母（

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公开/公告号CN105002192B

专利类型发明专利
公开/公告日2018-04-24

原文格式PDF
申请/专利权人昆明理工大学;
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申请/专利号CN201510434176.2
发明设计人张琦;崔锦锦;张慧君;季秀玲;魏云林;林连兵;
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申请日2015-07-23
分类号
代理机构
代理人
地址 650093 云南省昆明市五华区学府路253号
入库时间 2022-08-23 10:10:39

法律信息

法律状态公告日

法律状态信息

法律状态
2018-04-24

授权

授权
2015-11-25

实质审查的生效 IPC(主分类):C12N15/53 申请日:20150723

实质审查的生效
2015-10-28

公开

公开

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1. 一种苹果酸酶基因RKME1及其重组表达载体 [P] . 中国专利： CN105002192B . 2018.04.24
2. 一种苹果酸酶基因RKME2及其重组表达载体 [P] . 中国专利： CN105349557B . 2018.10.23
3. METHOD FOR PRODUCING A BACULOVIRUS VECTOR CAPABLE OF PRODUCING A SELECTED NON-FUSED RECOMBINANT PROTEIN OR CAPABLE OF BEING UTILIZED AS A TRANSFER VECTOR;A RECOMBINANT BACULOVIRUS EXPRESSION VECTOR CAPABLE OF EXPRESSING UNDER THE CONTROL OF A BACULOVIRUS POLYHEDRIN PROMOTER A SELECTED NON-FUSED PROTEIN IN A HOST INSECT CELL;A RECOMBINANT TRANSFER VECTOR UTILIZING A BACULOVIRUS POLYHEDRIN PROMOTER AND FLANKING SEQUENCES FOR INTRODUCING A SELECTED GENE INTO A BACULOVIRUS GENOME,OR FOR INSERTION OF A SELECTED GENE FOR A GENETIC MANIPULATION OF A BACULOVIRUS;AND A METHOD FOR THE SYNTHESIS OF A SELECTED NON-FUSED PROTEIN IN A HOST INSECT CELL USING THE EXPRESSION VECTOR [P] . IL71906A . 1992-08-18

机译：产生杆状病毒载体的方法，该载体能产生选择的非融合重组蛋白或能被用作转移载体;一种重组杆状病毒表达载体，其在一个条件下可以在特定的程序控制下进行表达宿主细胞;利用杆状病毒多角体蛋白启动子的重组转移载体，并将选择的基因引入杆状病毒基因组的侧翼序列，或将选择的基因插入用于基因的遗传操纵基因的方法和方法使用表达载体的宿主昆虫细胞中的非融合蛋白
4. Preparation of isolated protein, substantially pure or recombinant, isolated trt protein, substantially pure or recombinant isolated trt protein, substantially pure or recombinant, isolated trt protein or a variant thereof, or a fragment, polynucleotide, synthetic, substantially pure or recombinant, isolated nucleic acid, encoding a trt protein, expression vector, transfected cell, non-human animal, or its progeny, transgenic non-human animal, antibody or its binding fragment, antibody, specifically immunoreactive under immunologically reactive conditions, for a trt protein or its immunogenic fragment, processes of determining whether a compound or treatment is a modulator of a trt activity or expression, of determining whether a test compound is a modulator of a trt activity, of preparing recombinant telomerase, to detect a trt gene product in a sample, to detect the presence of d and at least one telomerase positive human cell in a biological sample comprising human cells, to diagnos [P] . BR9711844A . 2000-01-18

机译：分离的蛋白质，基本上纯的或重组的分离的trt蛋白，基本上纯的或重组的分离的trt蛋白，基本上纯的或重组的分离的trt蛋白或其变体或片段，多核苷酸，合成的，基本上纯的或重组的分离的核酸的制备编码trt蛋白的酸，表达载体，转染的细胞，非人类动物或其后代，转基因非人类动物，抗体或其结合片段，抗体，在免疫反应条件下对trt蛋白或其免疫原具有特异性片段，确定化合物或处理是否是trt活性或表达的调节剂，确定测试化合物是否是trt活性的调节剂，制备重组端粒酶以检测样品中trt基因产物的过程，检测包含人细胞的生物样品中d和至少一种端粒酶阳性人细胞的存在，以进行诊断
5. Insecticidally effective peptide, substantially isolated DNA sequence, recombinant expression vector, transgenic plant, recombinant baculovirus expression vector, recombinant host cell, processes for producing an insecticidally effective peptide and for controlling invertebrate pests, insecticidal composition, antibody, process for use antibodies, DNA probe, process to detect the presence of nucleic acid encoding an insecticide-effective peptide, prepro sequence, gene construction, promoter sequence and recombinant construction [P] . BR9205716A . 1994-06-07

机译：杀虫有效肽，基本分离的DNA序列，重组表达载体，转基因植物，重组杆状病毒表达载体，重组宿主细胞，生产杀虫有效肽和控制无脊椎动物有害生物的方法，杀虫组合物，抗体，抗体使用方法，DNA探针，检测编码杀虫有效肽的核酸，prepro序列，基因构建，启动子序列和重组构建的过程