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用于检测样品中的多个靶核酸序列的方法及装置

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摘要

本发明获取周期/信号变化值的数据集，由此，可在一个检测通道中有效检测多个靶核酸序列。

著录项

公开/公告号CN112004941A

专利类型发明专利
公开/公告日2020-11-27

原文格式PDF
申请/专利权人 SEEGENE株式会社;
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申请/专利号CN201980027488.8
发明设计人高成文;韩知慧;金英旭;朴永用;
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申请日2019-04-19
分类号C12Q1/6823(20060101);G16B40/10(20060101);
代理机构11038 中国贸促会专利商标事务所有限公司;
代理人郑天松
地址韩国首尔
入库时间 2023-06-19 09:01:25

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法律状态公告日

法律状态信息

法律状态
2023-10-27

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1. 用于检测样品中的多个靶核酸序列的方法及装置 [P] . 中国专利： CN112004941A . 2020-11-27
2. 一种用于鉴定样品中的含有靶核苷酸序列的一个或多个核酸分子的方法 [P] . 中国专利： CN113481281A . 2021-10-08
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途
5. methods for the synthesis of a nucleic acid molecule, to selectively amplify a target nucleic acid sequence of a DNA or a nucleic acid mixture, to amplify two or more target nucleotide sequences simultaneously, to sequence a target nucleic acid molecule , to detect a genetically diverse nucleic acid molecule, to detect a target nucleotide sequence in a nucleic acid sample, and to allow an annular specificity of an oligonucleotide to be determined by means of an oligonucleotide structure, and, oligonucleotide dual specificity [P] . BRPI0609031A2 . 2010-11-16

机译：合成核酸分子，选择性扩增DNA或核酸混合物的靶核酸序列，同时扩增两个或多个靶核苷酸序列，对靶核酸分子测序，检测遗传多样性的方法核酸分子，以检测核酸样品中的靶核苷酸序列，并允许通过寡核苷酸结构确定寡核苷酸的环状特异性，以及寡核苷酸双重特异性