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一种中华沙鳅DNA条形码序列及其应用

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摘要

本发明公开了一种中华沙鳅DNA条形码序列及其应用，属于物种鉴定技术领域。该所述中华沙鳅DNA条形码为中华沙鳅COI基因，该DNA条形码序列可以作为中华沙鳅DNA的标准检测序列，有效的对中华沙鳅特有种进行鉴定。

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公开/公告号CN108441564A

专利类型发明专利
公开/公告日2018-08-24

原文格式PDF
申请/专利权人江汉大学;
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申请/专利号CN201711214887.4
发明设计人刘红艳;熊飞;王莹;
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申请日2017-11-28
分类号C12Q1/6888(20180101);C12Q1/686(20180101);C12N15/11(20060101);
代理机构11302 北京华沛德权律师事务所;
代理人房德权
地址 430056 湖北省武汉市沌口经济技术开发区新江大路8号
入库时间 2023-06-19 06:17:24

法律信息

法律状态公告日

法律状态信息

法律状态
2018-09-18

实质审查的生效 IPC(主分类):C12Q1/6888 申请日:20171128

实质审查的生效
2018-08-24

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1. 一种中华沙鳅DNA条形码序列及其应用 [P] . 中国专利： CN108441564A . 2018-08-24
2. 一种红尾荷马条鳅DNA条形码序列及其应用 [P] . 中国专利： CN109055406A . 2018-12-21
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
4. Method for identifying the site of insertion of a DNA segment whose sequence is known, inserted in a stretch of DNA genu00c8mico ignored the site of a body.Method for determining the sequence of a stretch of DNA genu00c8mico unknown of a body in the vicinity of a stretch known,The preparation to identify the site of insertion of a DNA segment whose sequence is known, inserted in an unknown place in genu00c8mico DNA of an organism.Kit to identify the site of insertion of a DNA segment whose sequence is known, inserted in an unknown place in the DNA genu00c8mico a body and use of said preparation. [P] . BR0300337A . 2004-11-03

机译：鉴定已知序列的DNA片段的插入位点的方法，插入一段DNA gen的序列中的方法忽略了身体的位置。确定一段未知的DNA gen的序列的方法一种已知序列的制备方法，用于鉴定序列已知的DNA片段的插入位点的制剂，插入到生物体基因组DNA的未知位置。试剂盒用于鉴定其DNA片段的插入位点该序列是已知的，插入人体的DNA基因的未知位置，并使用所述制剂。
5. A process for isolating and purifying vasopeptidase-inhibiting peptides with specificity for the angiotensin-converting enzyme carboxylic site secreted by snake venom glands (bpps), particularly jararaca bothrops, or endogenously produced (evasins) having vasodilatory and anti- hypertensive; process of determining the starch sequence of inhibitor peptides secreted by the snake venom (bpps) or endogenous (evasin) venom; The process of determining the amino acid sequence of bpps by deducting the cdna from precursors of these molecules expressed in snake tissues, specifically bothrops jararaca. process of determining the amino acid sequence of evasins by deducing cdna from precursors of these molecules expressed in snake tissues, specifically bothrops jararaca, process of amplifying cdna from pancreatic and / or snake brain cdna libraries, specifically bothrops jararaca ; solid phase synthesis process of vasopeptidase inhibiting peptides with vasodilating and antihypertensive action, vaso [P] . BR0101088A . 2003-03-18

机译：一种分离和纯化具有对蛇毒腺（bpps），特别是贾拉卡双峰植物分泌的或具有血管扩张和抗高血压作用的内源性（evasins）分泌的血管紧张素转化酶羧基位点具有特异性的血管肽酶抑制肽的方法;确定蛇毒（bpps）或内源性（evasin）毒液分泌的抑制剂肽的淀粉序列的过程;通过从蛇组织中表达的这些分子的前体中减去cdna来确定bpps氨基酸序列的过程，尤其是杂种植物。通过从在蛇组织中表达的这些分子的前体推导cdna来确定evasins氨基酸序列的过程，特别是在蛇和蛇脑cdna文库中扩增cdna的过程，尤其是在蛇和草cdna文库中;血管肽酶抑制肽具有血管舒张和降压作用的固相合成方法