Application of ITS2 Sequence as DNA Barcode in Xanthium

摘要

[Objective]Xanthium plants( non-Chinese species) are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium species using ITS2 sequence as DNA barcode.[Method]ITS2 gene was amplified using universal primers to obtain ITS2 sequences of seven Xanthium species,which were then sequenced. ITS2 sequences of seven Xanthium species were compared and analyzed by MEGA 5. 1 software,and the phylogenetic tree was constructed.[Result]A total of 242 variable sites were found in ITS2 sequences of Xanthium species,including 12 singleton variable sites.[Conclusion]Xanthium species can be distinguished and identified based on ITS2 sequences.