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检测样品中KRAS基因的突变的方法

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摘要

提供检测样品中KRAS基因的突变的方法，本发明通过构建包含能够特异性识别并结合至野生型KRAS基因的待测靶序列的结合物的DNA扩增反应体系，对待检测靶序列进行扩增，并通过判断是否获得与待测靶序列对应的扩增产物，来判断该待测靶序列上是否存在突变。一次检测完成对KRAS基因待测靶序列上多种点突变的检测，简化了检测流程，降低了DNA起始用量，检测灵敏度0.5％。

著录项

公开/公告号CN106978495A

专利类型发明专利
公开/公告日2017-07-25

原文格式PDF
申请/专利权人元码基因科技(北京)有限公司;
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申请/专利号CN201710271601.X
发明设计人刘彦哲;梁羽;
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申请日2017-04-24
分类号C12Q1/68(20060101);
代理机构北京科石知识产权代理有限公司;
代理人高元吉;朱春野
地址 100102 北京市朝阳区广顺北大街5号融创动力A区4层
入库时间 2023-06-19 02:52:30

法律信息

法律状态公告日

法律状态信息

法律状态
2017-12-08

著录事项变更 IPC(主分类):C12Q1/68 变更前: 变更后: 申请日:20170424

著录事项变更
2017-08-18

实质审查的生效 IPC(主分类):C12Q1/68 申请日:20170424

实质审查的生效
2017-07-25

公开

公开

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1. 检测样品中KRAS基因的突变的方法 [P] . 中国专利： CN106978495A . 2017-07-25
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3. isolated polynucleotide, expression cassette, host cell, isolated polypeptide, non-human transgenic animal that has been altered to express an alkali resistant mutant polypeptide, an antibody that specifically binds to an alkali resistant mutant polypeptide, a mutation detection kit alk inhibitor resistance in a biological sample, and methods for evaluating a biological sample for an alk inhibitor resistance mutation to diagnose cancer that is resistant to or likely to develop resistance to at least one alkaline inhibitor. small alk molecule kinase, to evaluate a biological sample for an alk inhibitor resistance mutation, to diagnose cancer that is resistant to or likely to develop resistance to pf-02341066 in a patient, to specifically reduce expression of an alk-resistant mutant to treat cancer associated with activating aberrant loop capacity that is resistant to at least one small molecule alk kinase inhibitor treating an aberrant cancer that is resistant to pf-02341066, identifyin [P] . BR112012026239A2 . 2018-06-26

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4. Polinucleotu00eddeo insulated tape of expression, host cell, isolated transgenic non-human animal polypeptide, which has been modified to express a polypeptide mutant with resistance to ALK, antibody that binds specifically to a polypeptide mutant with resistance to ALK, kit p For detection of a mutation with resistance to ALK inhibitor in a biological sample, and,Methods for evaluating a biological sample as a mutation with resistance to ALK inhibitor, for diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to evaluate a biological sample that Anto a mutation with resistance to ALK inhibitor.For diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to assess the biological sample as a mutation with resistance to ALK inhibitor, a cancer that is resistant to dianosticar Or is likely to develop resistance to PF - 02341066 in a pati [P] . BR112012026239A2 . 2016-11-29

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5. DETECTION OF KRAS MUTATION IN EXON 2 BY ALLELE SPECIFIC REAL TIME QUANTITATIVE PCR (AS-QPCR) [P] . 世界知识产权组织专利： WO2011104695A3 . 2012-03-01

机译：等位基因特异性实时定量PCR（AS-QPCR）检测外显子2中的KRAS突变