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Repeated batch for dye degradation in an airlift bioreactor by laccase entrapped in copper alginate

机译:海藻酸铜包埋的漆酶在气浮式生物反应器中降解染料的重复批次

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摘要

A repeated batch of synthetic dye decolorization was efficiently demonstrated in a 5 L airlift bioreactor. A laccase from Ganoderma sp. KU-Alk4, degrading commercial aromatic dyes was selected. The crude enzyme extract expressed laccase activity, and was immobilized under optimal conditions in copper-alginate beads, 3 IU/bead. The immobilized enzyme showed high efficiency in degrading various synthetic dyes under non-buffered conditions, in particular the indigoid dye Indigo Carmine. The immobilized laccase also showed marked increase in stability toward temperature and pH when compared with free enzyme preparation. Immobilization enhanced its temperature stability to maintain initial activity up to 55 °C, ten degrees higher than the free enzyme. The immobilized laccase was stable in the alkaline region up to pH 10.0. The dye decolorization system in 5 L airlift bioreactor was demonstrated with 25 mg/L Indigo Carmine dissolved in tap water and a total immobilized laccase activity of 6 × 104 IU. Airflow rate was the most important factor affecting the number of batch runs and the time for 100% dye degradation. An optimal airflow rate was of 4 L/min. Fourteen batch runs of complete dye degradation were successfully completed with only a single enzyme supplementation, and this could be a feasible system for operation in industry. Total dye degraded by this repeated process at 4 L/min airflow rate was 1.8 g. Isatin sulfonic acid was a metabolic product of Indigo Carmine degradation catalyzed by the immobilized laccase. This development of an effective repeatable bioprocess using enzymes for the treatment of dye-contaminated effluent has potential for implementation on an industrial scale.
机译:在5 L气举式生物反应器中有效地证明了合成染料脱色的重复批次。一种来自灵芝的漆酶。选择了可降解的商用芳香族染料KU-Alk4。粗酶提取物表达漆酶活性,并在最佳条件下固定在3 IU /珠的海藻酸铜珠中。固定化酶在非缓冲条件下显示出高效率降解各种合成染料,特别是靛类染料靛蓝胭脂红。与游离酶制剂相比,固定化的漆酶还显示出对温度和pH的稳定性显着提高。固定化增强了其温度稳定性,以维持高达55°C的初始活性,比游离酶高十度。固定化的漆酶在碱性范围内稳定至pH 10.0。在自来水中溶解了25 mg / L靛蓝胭脂红和固定的漆酶总活性为6×104 IU后,证明了5 L气举式生物反应器中的染料脱色系统。气流速率是影响批生产次数和100%染料降解时间的最重要因素。最佳气流速率为4 L / min。仅需添加一种酶就可以成功完成十四次染料完全降解的分批运行,这对于工业操作而言可能是一个可行的系统。通过该重复过程以4 L / min的空气流速降解的总染料为1.8 g。伊斯兰磺酸是固定化漆酶催化的靛蓝胭脂红降解的代谢产物。使用酶来处理染料污染的废水的有效可重复生物过程的这种发展具有在工业规模上实施的潜力。

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