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A brackishwater isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems

机译:假单胞菌ps-102的半咸水分离物,一种针对对虾和非对虾饲养系统中致病性弧菌的潜在拮抗细菌

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摘要

A Pseudomonas sp PS-102 recovered from Muttukkadu brackish water lagoon, situated south of Chennai, showed significant activity against a number of shrimp pathogenic vibrios. Out of the 112 isolates of bacterial pathogens comprising Vibrio harveyi, V. vulnificus, V. parahaemolyticus, V. alginolyticus, V. fluvialis, and Aeromonas spp, 73% were inhibited in vitro by the cell-free culture supernatant of Pseudomonas sp PS-102 isolate. The organism produced yellowish fluorescent pigment on King's B medium, hydrolysed starch and protein, and produced 36.4% siderophore units by CAS assay and 32 μM of catechol siderophores as estimated by Arnow's assay. The PS-102 isolate showed wide ranging environmental tolerance with, temperatures from 25 to 40 °C, pH from 6 to 8, salinity from 0 to 36 ppt, while the antagonistic activity peaked in cultures grown at 30 °C, pH 8.0 and at 5 ppt saline conditions. The antagonistic activity of the culture supernatant was evident even at 30% v / v dilution against V. harveyi. The preliminary studies on the nature of the antibacterial action indicated that the antagonistic principle as heat stable and resistant to proteolytic, lipolytic and amylolytic enzymes. Pseudomonas sp PS 102 was found to be safe to shrimp when PL-9 stage were challenged at 107 CFU ml− 1 and by intramuscular injection into of 5 g sub-adults shrimp at 105 to 108 CFU. Further, its safety in a mammalian system, tested by its pathogenicity to mice, was also determined and its LD50 to BALB/c mice was found to be 109 CFU. The results of this study indicated that the organism Pseudomonas sp PS 102 could be employed as a potential probiont in shrimp and prawn aquaculture systems for management and control of bacterial infections.
机译:从位于金奈南部的Muttukkadu咸淡水泻湖中回收的假单胞菌sp-PS-102对许多虾病原性弧菌显示出显着活性。在112株细菌病原体中,包括哈维氏弧菌,创伤弧菌,副溶血弧菌,溶藻弧菌,河豚弧菌和气单胞菌,其中73%被假单胞菌PS-PS-的无细胞培养上清液抑制。 102株。该生物体在King's B培养基上产生了淡黄色的荧光色素,水解了淀粉和蛋白质,并通过CAS测定产生了36.4%的铁载体,通过Arnow分析估计产生了32μM的邻苯二酚铁载体。 PS-102分离株在25至40°C的温度,6至8的pH值,0至36 ppt的盐度下表现出广泛的环境耐受性,而拮抗活性在30°C,pH 8.0和80 5 ppt盐水条件。即使在30%v / v稀释下对哈维弧菌,培养上清液也具有明显的拮抗活性。对抗菌作用性质的初步研究表明,该拮抗作用原理是热稳定的,并且对蛋白水解,脂解和淀粉分解酶具有抵抗力。当在107 CFU ml-1挑战PL-9阶段并在105至108 CFU肌内注射5 g亚成年虾时,假单胞菌sp PS 102被发现对虾安全。此外,还确定了其在哺乳动物系统中的安全性,并通过对小鼠的致病性进行了测试,发现其对BALB / c小鼠的LD50为109 CFU。这项研究的结果表明,假单胞菌菌PS 102可作为虾和对虾水产养殖系统中潜在的益生菌用于细菌感染的管理和控制。

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