Proximate Composition, Chlorophyll a, and Carotenoid Content in Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae)udcultured with Cost-Effective Seaweed Liquid Fertilizer Medium

摘要

Growth, proximate composition, chlorophyll a, and carotenoid content were estimated in static cultures of micro algaudDunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) to compare the quality and quantity of biomass produced with seaweedudliquid fertilizer (SLF) against Walne’s medium. Culture of micro algae was performed with controlled temperature of 27 ± 1 °C,udsalinity of 33, and an irradiance of 120 ± 3 μ mol m–2 s –1, and daily samples were taken to estimate the above-mentioned parameters.udMean cellular density from samples of both medium were similar and growth rates were 0.44 and 0.42 doubling per day,udrespectively. Maximum values of protein content 5.2 and 5.5 pg per cell were estimated on day 5 in Walne’s medium and SLF,udrespectively. Carbohydrate and lipid content decreased during the first 4 days corresponding to the culture exponential growth.udHigher carbohydrate content was found in both media during the first 2 days and thereafter reduced partially compared to theirudconcentrations. Generally lipid contents in cultures with SLF were significantly higher (P ≤0.05) compared to Walne’s medium. Bothudpigments also increased exponentially and their concentration was same in both experiments. In conclusion, all the parameters testedudwere similar when using either media, and hence SLF can be used as an alternate media for micro algal culture.