首页> 外文OA文献 >Electrofusion of cells on chip: from a static, parallel approach to a high-througput, serial, microdroplet platform
【2h】

Electrofusion of cells on chip: from a static, parallel approach to a high-througput, serial, microdroplet platform

机译:芯片上细胞的电融合:从静态,并行方法到高通量,串行,微滴平台

摘要

In this thesis, the results of the development of two microfluidic devices (static and high­ throughput) for efficient electrofusion of cells were described and evaluated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. These hybridomas are the result of the fusion between a B-cell and a myeloma cell. The conventional method for generating hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microtluidic chips offer several advantages compared to the conventional systems.
机译:在这篇论文中,描述并评估了两种用于细胞高效电融合的微流体装置(静态和高通量)的开发结果。细胞的电融合是产生抗体产生杂交瘤的重要工具。这些杂交瘤是B细胞和骨髓瘤细胞之间融合的结果。产生杂交瘤的常规方法效率非常低(通常为0.001%左右)。结合对抗体日益增长的需求,这促使我们探索产生杂交瘤的更有效方法。我们认为,与常规系统相比,微流体芯片具有许多优势。

著录项

  • 作者

    Kemna Evelien;

  • 作者单位
  • 年度 2013
  • 总页数
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号