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>Electrofusion of cells on chip: from a static, parallel approach to a high-througput, serial, microdroplet platform
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Electrofusion of cells on chip: from a static, parallel approach to a high-througput, serial, microdroplet platform
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机译:芯片上细胞的电融合:从静态,并行方法到高通量,串行,微滴平台
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摘要
In this thesis, the results of the development of two microfluidic devices (static and high throughput) for efficient electrofusion of cells were described and evaluated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. These hybridomas are the result of the fusion between a B-cell and a myeloma cell. The conventional method for generating hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microtluidic chips offer several advantages compared to the conventional systems.
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