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A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel

机译:一种用于牙釉质仿生矿化的新型自组装寡肽两亲物

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摘要

Background Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA). Results The results showed that the OPA self-assembled into nano-fibres in the presence of calcium ions and in neutral acidity. The OPA was alternately immersed in calcium chloride and sodium hypophosphate solutions to evaluate its property of mineralization. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed nucleation and growth of amorphous calcium phosphate along the self-assembled OPA nano-fibres when it was repetitively exposed to solutions with calcium and phosphate ions. Energy dispersive spectrometry (EDS) confirmed that these nano-particles contained calcium and phosphate. Furthermore, electron diffraction pattern suggested that the nano-particles precipitated on OPA nano-fibres were comparable to amorphous calcium phosphate. Acid-etched human enamel slices were incubated at 37°C in metastable calcium phosphate solution with the OPA for biomimetic mineralization. SEM and X-ray diffraction indicated that the OPA induced the formation of hydroxyapatite crystals in organized bundles on etched enamel. TEM micrographs revealed there were 20–30 nm nano-amorphous calcium phosphate precipitates in the biomimetic mineralizing solution. The particles were found separately bound to the oligopeptide fibres. Biomimetic mineralization with or without the oligopeptide increased demineralized enamel microhardness. Conclusions A novel OPA was successfully fabricated, which fostered the biomimetic mineralization of demineralized enamel. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical therapy of dental erosion.
机译:背景技术研究人员正在寻找仿制的ena / mel仿生矿物质来控制牙齿侵蚀。这项研究评估了由合成和自组装的寡肽两亲物(OPA)诱导的脱矿质搪瓷的仿生矿化作用。结果结果表明,在钙离子存在和中性酸性下,OPA可自组装成纳米纤维。将OPA交替浸入氯化钙和次磷酸钠溶液中,以评估其矿化特性。透射电子显微镜(TEM)和扫描电子显微镜(SEM)显示,当无定形磷酸钙反复暴露于钙和磷酸根离子溶液中时,其沿自组装OPA纳米纤维的形核和生长。能量色散光谱法(EDS)证实这些纳米粒子包含钙和磷酸盐。此外,电子衍射图谱表明,沉淀在OPA纳米纤维上的纳米颗粒与无定形磷酸钙相当。将酸蚀后的人类牙釉质切片与OPA在亚稳态磷酸钙溶液中于37°C孵育,以进行仿生矿化。 SEM和X射线衍射表明,OPA在蚀刻的瓷釉上以有组织的束的形式诱导了羟基磷灰石晶体的形成。 TEM显微照片显示,仿生矿化溶液中存在20–30 nm的纳米非晶态磷酸钙沉淀。发现颗粒分别与寡肽纤维结合。有或没有寡肽的仿生矿化作用增加了脱矿质釉质的显微硬度。结论成功地制备了新型的OPA,其促进了脱矿质搪瓷的仿生矿化。这是为将来的牙侵蚀临床治疗设计和构建新型生物材料的主要步骤之一。

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