首页> 外文OA文献 >Complete mitochondrial genome of the Verticillium-wilt causing plant pathogen Verticillium nonalfalfae
【2h】

Complete mitochondrial genome of the Verticillium-wilt causing plant pathogen Verticillium nonalfalfae

机译:完成黄萎病的线粒体基因组导致植物病原体黄萎病菌(Verticillium nonalfalfae)

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Verticillium nonalfalfae is a fungal plant pathogen that causes wilt disease by colonizing the vascular tissues of host plants. The disease induced by hop isolates of V. nonalfalfae manifests in two different forms, ranging from mild symptoms to complete plant dieback, caused by mild and lethal pathotypes, respectively. Pathogenicity variations between the causal strains have been attributed to differences in genomic sequences and perhaps also to differences in their mitochondrial genomes. We used data from our recent Illumina NGS-based project of genome sequencing V. nonalfalfae to study the mitochondrial genomes of its different strains. The aim of the research was to prepare a V. nonalfalfae reference mitochondrial genome and to determine its phylogenetic placement in the fungal kingdom. The resulting 26,139 bp circular DNA molecule contains a full complement of the 14 "standard" fungal mitochondrial protein-coding genes of the electron transport chain and ATP synthase subunits, together with a small rRNA subunit, a large rRNA subunit, which contains ribosomal protein S3 encoded within a type IA-intron and 26 tRNAs. Phylogenetic analysis of this mitochondrial genome placed it in the Verticillium spp. lineage in the Glomerellales group, which is also supported by previous phylogenetic studies based on nuclear markers. The clustering with the closely related Verticillium dahliae mitochondrial genome showed a very conserved synteny and a high sequence similarity. Two distinguishing mitochondrial genome features were also found-a potential long non-coding RNA (orf414) contained only in the Verticillium spp. of the fungal kingdom, and a specific fragment length polymorphism observed only in V. dahliae and V. nubilum of all the Verticillium spp., thus showing potential as a species specific biomarker.
机译:苜蓿黄萎病菌是一种真菌植物病原体,通过在寄主植物的血管组织中定殖而引起枯萎病。由非苜蓿啤酒花分离株诱发的疾病表现为两种不同形式,分别从轻度症状到致死性致病菌引起的轻度症状到完全植物死亡。因果菌株之间的致病性差异已归因于基因组序列的差异,也可能归因于其线粒体基因组的差异。我们使用了来自最近基于Illumina NGS的基因组测序紫苜蓿的基因组数据,来研究其不同菌株的线粒体基因组。该研究的目的是制备非苜蓿弧菌参考线粒体基因组,并确定其在真菌界的系统发生位置。产生的26,139 bp环状DNA分子包含电子传输链和ATP合酶亚基的14个“标准”真菌线粒体蛋白编码基因的完整互补,以及一个小的rRNA子单元,一个大的rRNA子单元,其中包含核糖体蛋白S3在IA型内含子和26种tRNA中编码的。对线粒体基因组进行系统发育分析,将其置于黄萎病菌中。 Glomerellales组的血统,先前的基于核标记的系统发育研究也支持这一点。与紧密相关的黄萎病线粒体基因组的聚类显示非常保守的同义性和高度的序列相似性。还发现了两个不同的线粒体基因组特征-仅在黄萎病菌中含有潜在的长非编码RNA(orf414)。真菌王国,并且仅在所有黄萎病菌的黄萎病菌和裸孢菌中观察到特定的片段长度多态性,因此显示出作为物种特异性生物标记物的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号