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Effects of secondary compounds from cactus and acacias trees on rumen microbial profile changes performed by Real-Time PCR

机译:实时pCR研究仙人掌和金合欢树次生化合物对微生物谣传微生物形态变化的影响

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摘要

Plant rich secondary compounds had antimicrobial effects by acting against different rumen microbial populations. The current study investigated the influence of spineless cactus (Opuntia ficus indica f. inermis), Acacia nilotica and A. saligna on rumen microbial fermentation, using in vitro gas production technique, and microbial population profile changes, using a molecular-based technique (Real-Time PCR). The acacias and Opuntia reduced significantly total gas production (p0.01), rumen CH4 production (p?0.01) and ammonia concentration (p0.001). At 24h of incubation, Fungi population was 0.30- and 0.03 -fold reduced with A.nilotica and Opuntia as compared to 0h, but 2-and 1.24- fold higher with A.cyanophylla .Increases in the abundance of F.succinogenes were observed in all substrates; however, the tanniferous plants and Opuntia reduced the relative abundance of R.flavefaciens. Methanogenic population was increased with all substrates, except for Opuntia (0. 90- fold lower than the control). There was a significant reduction (p0.05) in rumen protozoa count with A.cyanophylla, Opuntia and A.nilotica (3.68; 5.59 and 5.34 times, respectively). Results suggested that tannin sources from A.nilotica and A.cyanophylla had an indirect effect on methanogenesis. This study showed an antimicrobial activity of oxalates content of O. ficus indica.
机译:富含植物的次要化合物通过对抗不同的瘤胃微生物种群而具有抗菌作用。当前的研究使用体外产气技术研究了无脊柱仙人掌(Opuntia ficus indica f。inermis),Acacia nilotica和A.saligna对瘤胃微生物发酵的影响,以及使用基于分子的技术(Real -时间PCR)。相思和仙人掌显着降低了总产气量(p <0.01),瘤胃CH4产生量(p <0.01)和氨浓度(p <0.001)。孵化24h时,与0h相比,A. nilotica和Opuntia的真菌种群减少了0.30和0.03倍,而A.cyanophylla则使真菌种群增长了2和1.24倍。所有基材;但是,单宁植物和仙人掌减少了黄萎病菌的相对丰度。除仙人掌外,所有底物的产甲烷菌种群均增加(比对照低0. 90倍)。蓝藻,仙人掌和尼罗罗非鱼的瘤胃原生动物数量显着减少(p <0.05)(分别为3.68、5.59和5.34倍)。结果表明,来自A.nilotica和A.cyanophylla的单宁来源对甲烷生成有间接影响。这项研究表明O草中草酸盐含量的抗菌活性。

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