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Filamentous fungi in drinking water tanks of a water supply system in Recife-PE, Brazil

机译:巴西累西腓PE供水系统饮用水箱中的丝状真菌

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摘要

Drinking water reservoirs are widely used in various building constructions inBrazil for as a source of potable water for users. However, they are susceptible tothe growth of microorganisms such as fungi. From these, several negative factorsmay ensue, namely, unpleasant odors and flavours, pigments, biofilm formationand mycotoxins. This is caused fungal resistance to treatment and disinfection.Brazilian law and those of other countries offer no encouragement for the researchof the species of fungi that cause these problems. There are no legal limits as tothe presence in drinking water. This study assesses the occurrence of filamentousfungi in drinking water reservoirs connected to the Alto do Céu distribution systemlocated in Recife, Pernambuco, Brazil. Four monthly collections were carried out,in triplicate, at two fixed points in the network, at the same treatment area, andanother 2.0 km away from this site. For water physicochemical characterization atthe time of collection, pH, temperature and free residual chlorine were analyzed.Filamentous fungi were quantified by filtration through cellulose nitrate membranewith a 0.45 μm porosity. All strains were grown in Petri dishes containing PeptoneGlucose, Rose Bengal Agar (PGRBA) and were incubated at 30°C for 48h. Fungaloccurrence was quantified as colony forming units (CFU) per litre. Twenty-foursamples were analyzed, and these accounted for 807 CFU. Considering the potentialfor water spreading diseases, and the high toxicity of fungi into account it isintended that this work may prompt the creation of acceptable parameters as tothe presence of fungi in drinking water networks.
机译:饮用水水库广泛用于巴西的各种建筑中,作为用户的饮用水来源。但是,它们对诸如真菌的微生物的生长敏感。由此,可能会产生一些负面因素,即令人不愉快的气味和风味,色素,生物膜形成和霉菌毒素。这导致了真菌对治疗和消毒的抗性。巴西法律和其他国家/地区的法律不鼓励对引起这些问题的真菌种类进行研究。饮用水中没有法律限制。这项研究评估了与位于巴西伯南布哥累西腓的Alto doCéu分配系统相连的饮用水水库中丝状真菌的发生。在网络中的两个固定点,相同的治疗区域以及距该站点另外2.0公里处,一式三份地进行了四个月度收集。为了收集时的水理化特性,分析pH值,温度和游离残留氯。通过孔径为0.45μm的硝酸纤维素膜过滤来定量丝状真菌。所有菌株均在含有蛋白G葡萄糖,玫瑰孟加拉琼脂(PGRBA)的培养皿中生长,并在30°C下孵育48h。真菌的发生被量化为每升菌落形成单位(CFU)。分析了24个样本,这些样本占807 CFU。考虑到潜在的水传播疾病,以及真菌的高毒性,预期这项工作可能会促使人们建立饮用水网络中真菌存在的可接受参数。

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