Microbiological analyses of eggs and egg products using the MicroFoss system

摘要

This thesis evaluates the MicroFoss system, also known as the BioSys or the SolerisÒ system, as a rapid method for microbiological analyses of eggs and egg products. Commercially assays for assessment of microbiological quality and sanitation conditions during processing, which are total viable counts (TVC), coliforms and Escherichia coli, are evaluated in comparison to the cultural methods. Concurrently, this thesis reports development of a new medium for the MicroFoss Salmonella detection, since this test is not available.Good correlations between the cultural TVCs and the MicroFoss detection times (DTs) were obtained (r ³-0.84) in all egg products. Preliminary results of a correlation between the cultural TVCs dominated by Pseudomonas fluorescens and the MicroFoss DTs suggested that the MicroFoss could be used for investigation of spoilage and shelf life prediction of eggs.High agreements (r ³-0.93) between the MicroFoss and cultural methods for coliforms and E. coli in eggs and egg products was achieved as well as the accuracy, sensitivity and specificity. The MicroFoss for estimations of TVC, coliforms and E. coli could be completed in less than 24h, compared to 48-72h required for the cultural methods. Hence, the MicroFoss could be used as a rapid screening for quality control of eggsand egg products.While a new propylene glycol-based selective medium developed was effective for detection of Salmonella, possible false positive results caused by certain bacteria, such as Enterobacter cloacae, E. coli, Klebsiella pneumoniae and Proteus mirabilis, were encountered. When this selective medium was supplemented with 40 mg/L of novobiocin, or 1 or 2 mL/L of Niaproof 4, these non-salmonellae were greatly suppressed or eliminated, particularly Pr. mirabilis, whilst this did not affect growth of Salmonella. Various serotypes of Salmonella including strains of atypical biotype were able to acidify propylene glycol in these media.Detection of Salmonella in naturally contaminated eggs by the MicroFoss using propylene glycol-based selective media showed equivalent sensitivity and specificity to the Australian Standard 5013.10-2004, according to numbers of egg samples tested in this study. Detection of low level of Salmonella (1-10 CFU/25g of eggs pre-enriched in buffered peptone water) was achieved in less than 24h.