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Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.

机译:百日咳博德特氏菌aroA基因的克隆和核苷酸序列。

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摘要

The aroA locus of Bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into Escherichia coli by using a cosmid vector. The gene is expressed in E. coli and complemented an E. coli aroA mutant. The nucleotide sequence of the B. pertussis aroA gene was determined and contains an open reading frame encoding 442 amino acids, with a calculated molecular weight for 5-enolpyruvylshikimate 3-phosphate synthase of 46,688. The amino acid sequence derived from the nucleotide sequence shows homology with the published amino acid sequences of aroA gene products of other microorganisms.
机译:百日咳博德特氏菌的aroA基因座(编码5-烯丙基丙酮酸shi草酸酯3-磷酸合酶)已通过使用粘粒载体克隆到大肠杆菌中。该基因在大肠杆菌中表达,并与大肠杆菌aroA突变体互补。确定了百日咳博德特氏菌aroA基因的核苷酸序列,并包含一个编码442个氨基酸的开放阅读框,其5-烯丙基丙酮酸shi草酸酯3-磷酸合酶的计算分子量为46,688。来源于核苷酸序列的氨基酸序列与其他微生物的aroA基因产物的公开氨基酸序列具有同源性。

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