首页> 外文OA文献 >Identification and Manipulation of Soil Properties To Improve the Biological Control Performance of Phenazine-Producing Pseudomonas fluorescens
【2h】

Identification and Manipulation of Soil Properties To Improve the Biological Control Performance of Phenazine-Producing Pseudomonas fluorescens

机译:鉴定和处理土壤性质以提高生产吩嗪类荧光假单胞菌的生物防治性能

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Pseudomonas fluorescens 2-79RN10 protects wheat against take-all disease caused by Gaeumannomyces graminis var. tritici; however, the level of protection in the field varies from site to site. Identification of soil factors that exert the greatest influence on disease suppression is essential to improving biocontrol. In order to assess the relative importance of 28 soil properties on take-all suppression, seeds were treated with strain 2-79RN10 (which produces phenazine-1-carboxylate [PCA+]) or a series of mutants with PCA+ and PCA− phenotypes. Bacterized seeds were planted in 10 soils, representative of the wheat-growing region in the Pacific Northwest. Sixteen soil properties were correlated with disease suppression. Biocontrol activity of PCA+ strains was positively correlated with ammonium-nitrogen, percent sand, soil pH, sodium (extractable and soluble), sulfate-sulfur, and zinc. In contrast, biocontrol was negatively correlated with cation-exchange capacity (CEC), exchangeable acidity, iron, manganese, percent clay, percent organic matter (OM), percent silt, total carbon, and total nitrogen. Principal component factor analysis of the 16 soil properties identified a three-component solution that accounted for 87 percent of the variance in disease rating (biocontrol). A model was identified with step-wise regression analysis (R2 = 0.96; Cp statistic = 6.17) that included six key soil properties: ammonium-nitrogen, CEC, iron, percent silt, soil pH, and zinc. As predicted by our regression model, the biocontrol activity of 2-79RN10 was improved by amending a soil low in Zn with 50 μg of zinc-EDTA/g of soil. We then investigated the negative correlation of OM with disease suppression and found that addition of OM (as wheat straw) at rates typical of high-OM soils significantly reduced biocontrol activity of 2-79RN10.
机译:荧光假单胞菌2-79RN10可保护小麦免受由Gaeumannomyces graminis var引起的全盘侵染。小麦但是,现场的保护级别因站点而异。识别对疾病抑制影响最大的土壤因素对于改善生物防治至关重要。为了评估28种土壤性质对全面抑制的相对重要性,用2-79RN10菌株(产生吩嗪-1-羧酸[PCA +])或一系列具有PCA +和PCA-表型的突变体处理了种子。细菌化的种子种植在10个土壤中,代表了西北太平洋地区的小麦种植区。十六种土壤特性与疾病抑制相关。 PCA +菌株的生物防治活性与铵态氮,沙子百分比,土壤pH,钠(可萃取和可溶),硫酸盐硫和锌呈正相关。相反,生物防治与阳离子交换能力(CEC),可交换酸度,铁,锰,粘土百分比,有机物百分比(OM),粉沙百分比,总碳和总氮呈负相关。对16种土壤特性的主成分因子分析确定了三成分解决方案,该解决方案占疾病等级差异的87%(生物防治)。通过逐步回归分析(R2 = 0.96; Cp统计= 6.17)确定了一个模型,该模型包括六个关键的土壤特性:铵氮,CEC,铁,淤泥百分比,土壤pH和锌。正如我们的回归模型所预测的,通过用50μg锌-EDTA / g的土壤改良低锌的土壤,可以提高2-79RN10的生物防治活性。然后,我们调查了OM与疾病抑制的负相关性,发现以高OM土壤的典型速率添加OM(作为麦秸)显着降低了2-79RN10的生物防治活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号