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Identification of a Novel T-Cell Epitope in Soluble Egg Antigen of Schistosoma japonicum

机译:日本血吸虫可溶性卵抗原中新型T细胞表位的鉴定

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摘要

Identification of T-cell epitopes harbored in soluble egg antigen (SEA) of Schistosoma japonicum and study of the immunological properties are essential for understanding the immunopathology and the control of schistosomiasis. As a follow-up to our previous work, the 66- to 80-kDa fragment from SEA was partially digested with protease, fractionated by reverse-phase high-pressure liquid chromatography, and found to be carrying a peptide which stimulated proliferation and gamma interferon (IFN-γ) production of Th1 clones specific to SEA. Sequence analysis showed that the peptide was composed of 12 amino acids lined up as DLAVELAYLGNL. A synthetic homologue induced proliferation and IFN-γ and interleukin-2 (IL-2) production, but not IL-4 or IL-6 production, by the Th1 clones as well as by the spleen cells from SEA-immunized mice, thus indicating that the peptide carries a Th1 epitope of SEA.
机译:鉴定日本血吸虫可溶性卵抗原(SEA)中所含的T细胞表位和免疫学特性的研究对于了解免疫病理学和控制血吸虫病至关重要。作为我们先前工作的后续措施,将SEA的66-80kDa片段用蛋白酶部分消化,通过反相高压液相色谱分离,发现带有刺激增殖和伽马干扰素的肽。 (IFN-γ)生产SEA特异的Th1克隆。序列分析表明该肽由12个氨基酸组成,排列为DLAVELAYLGNL。合成的同源物通过Th1克隆以及来自SEA免疫小鼠的脾细胞诱导增殖以及IFN-γ和白介素2(IL-2)的产生,但不诱导IL-4或IL-6的产生,因此表明该肽带有SEA的Th1表位。

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