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Excitation and desensitization of mouse rod photoreceptors in vivo following bright adapting light

机译:明亮的适应光在体内对小鼠杆状感光体的激发和脱敏

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摘要

Electroretinographic (ERG) methods were used to determine response properties of mouse rod photoreceptors in vivo following adapting illumination that produced a significant extent of rhodopsin bleaching. Bleaching levels prevailing at ∼10 min and ∼20 min after the adapting exposure were on average 14% and 9%, respectively, based on the analysis of visual cycle retinoids in the eye tissues. Recovery of the rod response to the adapting light was monitored by analysing the ERG a-wave response to a bright probe flash presented at varying times during dark adaptation. A paired-flash procedure, in which the probe flash was presented at defined times after a weak test flash of fixed strength, was used to determine sensitivity of the rod response to the test flash. Recovery of the response to the adapting light was 80% complete at 13.5 ± 3.0 min (mean ± s.d.; n = 7) after adapting light offset. The adapting light caused prolonged desensitization of the weak-flash response derived from paired-flash data. By comparison with results obtained in the absence of the adapting exposure, desensitization determined with a test-probe interval of 80 ms was ∼fourfold after 5 min of dark adaptation and ∼twofold after 20 min. The results indicate, for mouse rods in vivo, that the time scale for recovery of weak-flash sensitivity substantially exceeds that for the recovery of circulating current following significant rhodopsin bleaching. The lingering desensitization may reflect a reduced efficiency of signal transmission in the phototransduction cascade distinct from that due to residual excitation.
机译:视网膜电图(ERG)方法用于确定适应性照明后产生的视紫红质脱色的程度,从而确定小鼠杆感光体的体内反应特性。根据对眼睛组织中视觉周期类视色素的分析,适应性暴露后约10分钟和约20分钟时占主导地位的漂白水平分别平均为14%和9%。通过分析在黑暗适应过程中不同时间出现的对亮探针闪光的ERG a波响应,来监测棒对适应光的响应的恢复。使用成对闪光程序,其中在固定强度的弱测试闪光后的指定时间显示探针闪光,用于确定棒对测试闪光的响应灵敏度。适应光偏移后,在13.5±3.0分钟(平均±s.d.; n = 7)下,对适应光的响应恢复了80%。适应光导致对源自成对闪光数据的弱闪光响应长时间失去敏感性。通过与没有适应性暴露的结果进行比较,在黑暗适应5分钟后,以80毫秒的测试探针间隔确定的脱敏度约为4倍,而在20分钟后约为2倍。结果表明,对于体内的鼠杆,视紫红质显着漂白后,恢复弱闪光敏感性的时间范围大大超过了恢复循环电流的时间范围。挥之不去的脱敏现象可能反映出光导级联中信号传输效率的降低,这与残留激发不同。

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