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Characterization, Seasonal Occurrence, and Diel Fluctuation of Poly(hydroxyalkanoate) in Photosynthetic Microbial Mats

机译:光合微生物垫中聚(羟基链烷酸酯)的表征,季节性发生和狄尔波动。

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摘要

In situ poly(hydroxyalkanoate) (PHA) levels and repeating-unit compositions were examined in stratified photosynthetic microbial mats from Great Sippewissett Salt Marsh, Mass., and Ebro Delta, Spain. Unlike what has been observed in pure cultures of phototrophic bacteria, the prevalence of hydroxyvalerate (HV) repeating units relative to hydroxybutyrate (HB) repeating units was striking. In the cyanobacteria-dominated green material of Sippewissett mats, the mole percent ratio of repeating units was generally 1HB:1HV. In the purple sulfur bacteria-dominated pink material the relationship was typically 1HB:2HV. In Sippewissett mats, PHA contributed about 0.5 to 1% of the organic carbon in the green layer and up to 6% in the pink layer. In Ebro Delta mats, PHA of approximately 1HB:2HV-repeating-unit distribution contributed about 2% of the organic carbon of the composite photosynthetic layers (the green and pink layers were not separated). Great Sippewissett Salt Marsh mats were utilized for more extensive investigation of seasonal, diel, and exogenous carbon effects. When the total PHA content was normalized to organic carbon, there was little seasonal variation in PHA levels. However, routine daily variation was evident at all sites and seasons. In every case, PHA levels increased during the night and decreased during the day. This phenomenon was conspicuous in the pink layer, where PHA levels doubled overnight. The daytime declines could be inhibited by artificial shading. Addition of exogenous acetate, lactate, and propionate induced two- to fivefold increases in the total PHA levels when applied in the daylight but had no effect when applied at night. The distinct diel pattern of in situ PHA accumulation at night appears to be related, in some phototrophs, to routine dark energy metabolism and is not influenced by the availability of organic nutrients.
机译:在来自马萨诸塞州Great Sippewissett Salt Marsh和西班牙埃布罗三角洲的分层光合微生物垫中,检查了原位聚(羟基链烷酸酯)(PHA)的水平和重复单元的组成。与在光养细菌的纯培养物中观察到的不同,羟戊酸酯(HV)重复单元相对于羟丁酸酯(HB)重复单元的流行是惊人的。在Sippewissett席子中以蓝细菌为主的绿色材料中,重复单元的摩尔百分比通常为1HB:1HV。在以紫色硫细菌为主的粉红色材料中,这种关系通常为1HB:2HV。在Sippewissett垫中,PHA在绿色层中贡献了约0.5%到1%的有机碳,在粉红色层中贡献了6%的有机碳。在埃布罗(Ebro)三角垫中,大约1HB:2HV重复单元分布的PHA贡献了复合光合层有机碳的2%(绿色和粉红色层未分离)。大Sippewissett盐沼垫子被用于更广泛地研究季节,diel和外源碳的影响。当将总PHA含量标准化为有机碳时,PHA水平几乎没有季节性变化。但是,在所有地点和季节,日常日常变化都很明显。在每种情况下,PHA含量在夜间都会升高,而在白天则降低。这种现象在粉红色层中尤为明显,其中PHA含量过夜增加了一倍。白天的下降可以通过人工阴影来抑制。当在白天使用时,外源乙酸盐,乳酸和丙酸酯的添加会导致总PHA水平增加2至5倍,而在晚上使用时则没有影响。夜间,原位PHA积累的明显diel模式似乎与某些暗养动物的日常暗能量代谢有关,并且不受有机养分利用率的影响。

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