首页> 外文OA文献 >Intrinsic bioactivity of thyrotropin in human serum is inversely correlated with thyroid hormone concentrations. Application of a new bioassay using the FRTL-5 rat thyroid cell strain.
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Intrinsic bioactivity of thyrotropin in human serum is inversely correlated with thyroid hormone concentrations. Application of a new bioassay using the FRTL-5 rat thyroid cell strain.

机译:人血清中促甲状腺素的内在生物活性与甲状腺激素浓度成反比。使用FRTL-5大鼠甲状腺细胞株的新生物测定法的应用。

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摘要

We have developed a new bioassay for thyrotropin (TSH) in human serum to evaluate bioactivity in normal individuals and patients with different degrees of primary hypothyroidism. Unpurified TSH in serum showed no stimulation of cyclic AMP production in cultured FRTL-5 rat thyroid cells, but after immunopurification showed potent stimulatory activity. Immunoaffinity purification permitted up to 400-fold concentration of serum TSH, allowing bioactivity measurements even in certain normal sera. The limit of detection in the FRTL-5 bioassay was 10 microU of human TSH per 0.5 ml incubate, and half-maximal responses for standard human TSH was 102 +/- 26 (+/- SE) microU/0.5 ml. Immunoaffinity-purified serum TSH varied in bioactivity-to-immunoactivity (B/I) ratios from less than 0.25 to 1.21 among four euthyroid subjects and eight primary hypothyroid patients. An inverse correlation was found between B/I ratios of immunopurified basal TSH and the serum-free T4 (r = -0.7237, P less than 0.01), T4 (r = -0.6650, P less than 0.05), and T3 (r = -0.6382, P less than 0.05). B/I ratios of immunopurified TSH from three hypothyroid patients before and after acute stimulation by thyrotropin-releasing hormone showed no significant change, despite major changes in serum TSH. In summary, the present study shows an inverse relationship between the metabolic status of an individual and the intrinsic bioactivity of TSH.
机译:我们已经开发了一种新的人血清中促甲状腺激素(TSH)的生物检测方法,以评估正常人和不同程度的原发性甲状腺功能减退症患者的生物活性。血清中未纯化的TSH在培养的FRTL-5大鼠甲状腺细胞中未显示出环AMP产生的刺激,但在免疫纯化后显示出强大的刺激活性。免疫亲和纯化可以使血清TSH浓度达到400倍,即使在某些正常血清中也可以进行生物活性测量。 FRTL-5生物测定法的检测极限是每0.5 ml培养液中10 microU人TSH,标准人TSH的半数最大响应为102 +/- 26(+/- SE)microU / 0.5 ml。免疫亲和纯化的血清TSH的生物活性与免疫活性(B / I)之比在0.25例甲状腺功能正常患者和8例原发性甲状腺功能减退患者中从0.25到1.21不等。免疫纯化的基础TSH的B / I比与无血清T4(r = -0.7237,P小于0.01),T4(r = -0.6650,P小于0.05)和T3(r = -0.6382,P小于0.05)。尽管促甲状腺激素释放激素在血清中发生了重大变化,但在甲状腺促甲状腺激素释放激素急性刺激之前和之后,来自三名甲状腺机能减退患者的免疫纯化促甲状腺激素的B / I比值均未见明显变化。总而言之,本研究显示了个体的代谢状态与TSH固有生物活性之间的反比关系。

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