首页> 外文OA文献 >ARIA, an Arabidopsis Arm Repeat Protein Interacting with a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, Is a Novel Abscisic Acid Signaling Component1
【2h】

ARIA, an Arabidopsis Arm Repeat Protein Interacting with a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, Is a Novel Abscisic Acid Signaling Component1

机译:ARIA,与脱落酸响应基因表达的转录调节剂相互作用的拟南芥手臂重复蛋白,是一种新型的脱落酸信号Component1。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Arabidopsis (Arabidopsis thaliana) genome contains more than 90 armadillo (arm) repeat proteins. However, their functions are largely unknown. Here, we report that an Arabidopsis arm repeat protein is involved in abscisic acid (ABA) response. We carried out two-hybrid screens to identify signaling components that modulate ABA-responsive gene expression. Employing a transcription factor, ABF2, which controls the ABA-dependent gene expression via the G-box type ABA-responsive elements, we isolated an arm repeat protein. The ABF2-interacting protein, designated as ARIA (arm repeat protein interacting with ABF2), has another conserved sequence motif, BTB/POZ (broad complex, tramtrak, and bric-a-brac/poxvirus and zinc finger) domain, in the C-terminal region. The physiological relevance of ABF2-ARIA interaction was supported by their similar expression patterns and similar subcellular localization. Plants overexpressing ARIA are hypersensitive to ABA and high osmolarity during germination and insensitive to salt during subsequent seedling growth. By contrast, an ARIA knockout mutant exhibits ABA and glucose insensitivities. Changes in the expression levels of several ABF2-regulated genes were also observed in ARIA overexpression lines, indicating that ARIA modulates the transcriptional activity of ABF2. Together, our data indicate that ARIA is a positive regulator of ABA response.
机译:拟南芥(Arabidopsis thaliana)基因组包含90多个犰狳(arm)重复蛋白。但是,它们的功能很大程度上未知。在这里,我们报告拟南芥手臂重复蛋白参与脱落酸(ABA)的反应。我们进行了两种杂交筛选,以鉴定调节ABA响应基因表达的信号传导成分。我们利用转录因子ABF2(通过G-box型ABA响应元件控制ABA依赖的基因表达)来分离臂重复蛋白。与ABF2相互作用的蛋白被称为ARIA(与ABF2相互作用的重复臂蛋白),在C区域具有另一个保守的序列基序BTB / POZ(宽复合体,tramtrak和bric-a-brac /痘病毒和锌指)域。 -末端区域。 ABF2-ARIA相互作用的生理相关性得到了它们相似的表达模式和相似的亚细胞定位的支持。过度表达ARIA的植物在发芽过程中对ABA高度敏感,并且在渗透率方面具有高渗透压,在随后的幼苗生长过程中对盐不敏感。相比之下,ARIA基因敲除突变体表现出ABA和葡萄糖不敏感性。在ARIA过表达株系中也观察到了几种ABF2调节基因的表达水平变化,表明ARIA调节了ABF2的转录活性。总之,我们的数据表明ARIA是ABA反应的积极调节剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号