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Central Nervous System Extracellular Matrix as a Therapeutic Bioscaffold for Central Nervous System Injury

机译:中枢神经系统细胞外基质作为中枢神经系统损伤的治疗生物支架。

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摘要

Traumatic central nervous system (CNS) injuries lack effective treatment options and typically result in irrecoverable tissue damage and lifelong neurologic impairment. An ideal therapeutic would provide structural support for axonal regrowth as well as modulate the default secondary injury associated with CNS injuries. Extracellular matrix (ECM) bioscaffolds derived by decellularization promoted functional remodeling in numerous non-CNS applications; however, there has been minimal investigation of this technology in the CNS. The objectives of this work were to evaluate the tissue specific properties of CNS-ECM in terms of (1) hydrogel characteristics and biochemical composition, (2) neurotrophic potential, and (3) ability to alter the innate immune response.udBioscaffolds composed of CNS-ECM were formed into injectable solutions that polymerize to form hydrogels at body temperature. Hydrogels derived from CNS-ECM were compared to a hydrogel form of a non-CNS ECM, urinary bladder matrix (UBM-ECM), using compositional analyses for retained ECM molecules, mechanical assessments for rheological and turbidimetric properties, and multiphoton microscopy to visualize in-vitro three-dimensional neurite outgrowth. ECM hydrogels from both tissue sources had mechanical properties similar to native CNS and supported three-dimensional neurite outgrowth. udCNS-ECM and UBM-ECM bioscaffold mediated alteration of the innate immune and neural stem cell response was interrogated in-vitro using macrophages and spinal cord stem cells (SPCs). While all ECM scaffolds evaluated decreased astrocyte differentiation, only UBM-ECM increased SPC neuronal differentiation. Bioscaffolds derived from both CNS and non-CNS tissue sources promoted a pro-repair macrophage phenotype as demonstrated through immunofluorescent results. Finally, CNS-ECM bioscaffolds were compared to UBM-ECM in a rat model of contusion spinal cord injury. Macrophage polarization was evaluated over 4 weeks and a histologic evaluation of the lesion site completed. While the ECM bioscaffolds did not improve functional recovery, pro-repair macrophages were found closely associated with the ECM injection sites.udThis body of work demonstrates CNS-ECM bioscaffolds can be isolated and are capable of minimally invasive injection, supporting neurite extension in-vitro, and modulating macrophage and stem cell responses. Future research is necessary to determine the added benefits that can be obtained when this technology is combined with others known to be beneficial for CNS tissue repair.ud
机译:创伤性中枢神经系统(CNS)损伤缺乏有效的治疗选择,通常会导致无法恢复的组织损伤和终身神经系统损伤。理想的治疗方法将为轴突再生提供结构支持,并调节与中枢神经系统损伤相关的默认继发性损伤。通过脱细胞作用衍生的细胞外基质(ECM)生物支架在许多非CNS应用中促进了功能重塑;但是,在CNS中对该技术的研究很少。这项工作的目的是根据(1)水凝胶特征和生化成分,(2)神经营养潜能以及(3)改变先天免疫应答的能力来评估CNS-ECM的组织特异性。将CNS-ECM制成可注射溶液,这些溶液在人体温度下聚合形成水凝胶。使用保留的ECM分子的成分分析,流变学和比浊特性的机械评估以及多光子显微镜对CNS-ECM衍生的水凝胶与非CNS ECM,膀胱基质(UBM-ECM)的水凝胶形式进行了比较-体外三维神经突生长。来自两种组织来源的ECM水凝胶均具有与天然CNS相似的机械性能,并支持三维神经突生长。 udCNS-ECM和UBM-ECM生物支架介导的先天免疫和神经干细胞反应的改变是使用巨噬细胞和脊髓干细胞(SPC)进行体外研究的。尽管所有ECM支架均评估了星形胶质细胞分化的减少,但只有UBM-ECM可以增加SPC神经元的分化。通过免疫荧光结果证实,源自CNS和非CNS组织来源的生物支架均促进了修复前巨噬细胞表型。最后,在挫伤性脊髓损伤的大鼠模型中,将CNS-ECM生物支架与UBM-ECM进行了比较。在4周内评估巨噬细胞极化,并完成病变部位的组织学评估。虽然ECM生物支架不能改善功能恢复,但发现修复前的巨噬细胞与ECM注射位点密切相关。 ud这项工作表明,CNS-ECM生物支架可以分离,并且能够进行微创注射,从而支持神经突的扩展。体外,并调节巨噬细胞和干细胞反应。确定该技术与已知对中枢神经系统组织修复有益的其他技术相结合时可获得的附加益处,需要进行进一步的研究。 ud

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    Medberry Christopher J.;

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  • 年度 2014
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