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Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research

机译:Good's缓冲液作为开发用于生物研究的自缓冲和生物相容性离子液体的基础

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摘要

This work reports a promising approach to the development of novel self-buffering and biocompatible ionic liquids for biological research in which the anions are derived from biological buffers (Good's buffers, GB). Five Good's buffers (Tricine, TES, CHES, HEPES, and MES) were neutralized with four suitable hydroxide bases (1-ethyl-3-methylimidazolium, tetramethylammonium, tetraethylammonium, and tetrabutylammonium) producing 20 Good's buffer ionic liquids (GB-ILs). The presence of the buffering action of the synthesized GB-ILs was ascertained by measuring their pH-profiles in water. Moreover, a series of mixed GB-ILs with wide buffering ranges were formulated as universal buffers. The impact of GB-ILs on bovine serum albumin (BSA), here used as a model protein, is discussed and compared with more conventional ILs using spectroscopic techniques, such as infrared and dynamic light scattering. They appear to display, in general, a greater stabilizing effect on the protein secondary structure than conventional ILs. A molecular docking study was also carried out to investigate on the binding sites of GB-IL ions to BSA. We further used the QSAR-human serum albumin binding model, log K(HSA), to calculate the binding affinity of some conventional ILs/GB-ILs to HSA. The toxicity of the GB and GB-ILs was additionally evaluated revealing that they are non-toxic against Vitro fischeri. Finally, the GB-ILs were also shown to be able to form aqueous biphasic systems when combined with aqueous solutions of inorganic or organic salts, and we tested their extraction capability for BSA. These systems were able to extract BSA with an outstanding extraction efficiency of 100% in a single step for the GB-IL-rich phase, and, as a result, the use of GB-IL-based ABS for the separation and extraction of other added-value biomolecules is highly encouraging and worthy of further investigation.
机译:这项工作报告了一种有前途的方法,用于开发用于生物学研究的新型自缓冲和生物相容性离子液体,其中阴离子来源于生物缓冲液(Good's缓冲液,GB)。用四种合适的氢氧化物碱(1-乙基-3-甲基咪唑鎓,四甲基铵,四乙基铵和四丁基铵)中和五种Good's缓冲液(Tricine,TES,CHES,HEPES和MES),产生20种Good's缓冲离子液体(GB-IL)。通过测量其在水中的pH-分布来确定合成的GB-IL的缓冲作用的存在。此外,将一系列具有宽缓冲范围的混合GB-IL配制为通用缓冲液。讨论了GB-IL对牛血清白蛋白(BSA)(此处用作模型蛋白)的影响,并与使用光谱技术(如红外和动态光散射)的更常规ILs进行了比较。通常,它们似乎对蛋白质二级结构表现出比常规IL更大的稳定作用。还进行了分子对接研究,以研究GB-IL离子与BSA的结合位点。我们进一步使用QSAR-人血清白蛋白结合模型,log K(HSA),来计算一些常规IL / GB-IL与HSA的结合亲和力。另外评估了GB和GB-IL的毒性,显示它们对费氏体外无毒。最后,当与无机或有机盐的水溶液结合使用时,GB-ILs也显示出能够形成水相的两相体系,我们测试了它们对BSA的提取能力。这些系统能够在富含GB-IL的相中一步提取出100%出色提取效率的BSA,结果,使用基于GB-IL的ABS分离和提取其他高附加值生物分子令人鼓舞,值得进一步研究。

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