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One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules

机译:一步制备氨基PEG修饰的聚甲基丙烯酸甲酯微芯片,用于生物分子的电泳分离

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摘要

A simple method for a chemical surface modification of poly(methyl methacrylate) (PMMA) microchips with amino-poly(ethyleneglycol) (PEG-NH(2)) by nucleophilic addition-elimination reaction was developed to improve the separation efficiency and analytical reproducibility in a microchip electrophoresis (MCE) analysis of biomolecules such as proteins and enantiomers. In our procedure, the PEG chains were robustly immobilized only by introducing an aqueous solution of PEG-NH(2) into the PMMA microchannel. The electroosmotic mobilities on the modified chips remained almost constant during 35 days with 37 runs without any recoating. The PEG-NH(2) modified chip provided a fast, reproducible, efficient MCE separation of proteins with a wide variety of isoelectric points within 15s. Furthermore, the application of the modified chip to affinity electrophoresis using bovine serum albumin gave a good chiral separation of amino acids.
机译:建立了一种通过亲核加成消除反应对氨基甲基聚乙二醇(PEG-NH(2))进行聚甲基丙烯酸甲酯(PMMA)微芯片化学表面改性的简单方法,以提高分离效率和分析重现性。对蛋白质和对映异构体等生物分子的微芯片电泳(MCE)分析。在我们的程序中,仅通过将PEG-NH(2)的水溶液引入PMMA微通道将PEG链牢固地固定。改性芯片上的电渗迁移率在35天内保持了几乎恒定,共进行了37次,没有任何重涂。 PEG-NH(2)修饰的芯片提供了快速,可重现,高效的MCE分离蛋白质,在15秒内具有多种等电点。此外,将修饰的芯片应用于使用牛血清白蛋白进行的亲和电泳中,可以很好地手性分离氨基酸。

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