首页> 外文OA文献 >Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions
【2h】

Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions

机译:无氮条件下葡萄固氮菌由甘油生产多羟基丁酸酯和藻酸酯

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Glycerol is an interesting feedstock for biomaterials such as biofuels and bioplastics because of its abundance as a by-product during biodiesel production. Here we demonstrate glycerol metabolism in the nitrogen-fixing species Azotobacter vinelandii through metabolomics and nitrogen-free bacterial production of biopolymers, such as poly-d-3-hydroxybutyrate (PHB) and alginate, from glycerol. Glycerol-3-phosphate was accumulated in A. vinelandii cells grown on glycerol to the exponential phase, and its level drastically decreased in the cells grown to the stationary growth phase. A. vinelandii also overexpressed the glycerol-3-phosphate dehydrogenase gene when it was grown on glycerol. These results indicate that glycerol was first converted to glycerol-3-phosphate by glycerol kinase. Other molecules with industrial interests, such as lactic acid and amino acids including γ-aminobutyric acid, have also been accumulated in the bacterial cells grown on glycerol. Transmission electron microscopy revealed that glycerol-grown A. vinelandii stored PHB within the cells. The PHB production level reached 33% per dry cell weight in nitrogen-free glycerol medium. When grown on glycerol, alginate-overproducing mutants generated through chemical mutagenesis produced 2-fold the amount of alginate from glycerol than the parental wild-type strain. To the best of our knowledge, this is the first report on bacterial production of biopolymers from glycerol without addition of any nitrogen source.
机译:甘油是生物材料生产过程中的副产物,因此是生物材料(如生物燃料和生物塑料)的一种有趣原料。在这里,我们通过代谢组学和无氮细菌生产生物聚合物(例如聚-d-3-羟基丁酸酯(PHB)和藻酸酯),从甘油中证明固氮菌葡萄固氮菌中的甘油代谢。在磷酸甘油上生长的A. vinelandii细胞中积累了甘油3-磷酸,而在生长到固定生长期的细胞中其含量急剧下降。当其在甘油上生长时,A。vinelandii也过表达3-磷酸甘油脱氢酶基因。这些结果表明,甘油激酶首先将甘油转化为3-磷酸甘油。具有工业价值的其他分子,例如乳酸和包括γ-氨基丁酸在内的氨基酸,也已经积累在甘油上生长的细菌细胞中。透射电子显微镜显示,甘油生长的A. vinelandii将PHB存储在细胞内。在无氮甘油培养基中,PHB生产水平达到每干细胞重量33%。当在甘油上生长时,通过化学诱变产生的藻酸盐高产突变体从甘油中产生的藻酸盐量是亲本野生型菌株的2倍。据我们所知,这是关于不添加任何氮源的情况下由甘油细菌生产生物聚合物的第一份报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号