NEW FLUOROMETRIC METHODS FOR THE DETERMINATION OF ALUMINUM, PYROPHOSPHATE, AND ADENOSINE 5u27-TRIPHOSPHATE IN BIOLOGICAL SYSTEMS.

摘要

A technique has been developed to determine ATP and pyrophosphate from one aliquot of platelets. The sample which contains both ATP and pyrophosphate was used to generate NAD$sp{+}$ through a sequence of enzymatic reactions starting with ATP. The NAD$sp{+}$ generated was condensed with acetone to form a fluorescent product. Prior to the formation of this product, pyrophosphate was separated from NAD$sp{+}$ by passage of the reaction mixture through a Dowex-1 (Cl$sp{-}$) resin and subsequent elution with HCl. Recoveries for both assays ranged from 98-106%, and coefficients of variation for both within-run and between-run studies for both assays were below 7%.Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses u26 Major Papers - Basement, West Bldg. / Call Number: Thesis1987 .S387. Source: Dissertation Abstracts International, Volume: 48-10, Section: B, page: 2962. Thesis (Ph.D.)--University of Windsor (Canada), 1987.