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Phylogenetic analysis of bacterial communities in the shrimp and sea cucumber aquaculture environment in northern China by culturing and PCR-DGGE

机译:中国北方虾和海参养殖环境中细菌群落的系统发育分析-PCR-DGGE

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摘要

In this study, we investigated the bacterial communities in the shrimp and sea cucumber culture environment, including shrimp ponds (SP), sea cucumber ponds (SCP), mixed-culture ponds (MCP) and the effluent channel (EC) in Qingdao, China. Bacteria cultivation showed that the counts of heterotrophic, nitrate-reducing and sulfate-reducing bacteria in the sediment of SP were higher than that in the sediment of SCP and MCP, varying between 8.7 \ud7 10[4] and 1.86 \ud7 10[6], 2.1 \ud7 10[4] and 1.1 \ud7 10[5], and 9.3 \ud7 10[1] and 1.1 \ud7 10[4] CFU g-1, respectively. In contrast, the counts of ammonium-oxidizing and nitrifying bacteria in the sediment of SP was lower than that in the sediment of SCP and MCP. Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA gene and dendrogram analyses showed that bacterial diversity in the mixed-culture environment was higher than that in the monocultures. The similarity of bacterial community between EC and SCP or MCP was higher than that between EC and SP. These results indicated that sea cucumber culture played a significant role in influencing the environmental bacterial communities that were composed mainly of Flavobacteriaceae (64.3%), Bacteriodetes (21%) and delta proteobacteria (14.7%), including the genera of Croceimarina, Lutibacter, Psychroserpens and so on. The results explained the benefit of sea cucumber culture in shrimp ponds at the level of microbial ecology.
机译:在这项研究中,我们调查了中国青岛市虾和海参养殖环境中的细菌群落,包括虾池(SP),海参池(SCP),混合养殖池(MCP)和出水通道(EC)。 。细菌培养表明,SP沉积物中的异养细菌,减少硝酸盐和减少硫酸盐的细菌数量高于SCP和MCP沉积物中的细菌,在8.7 \ ud7 10 [4]和1.86 \ ud7 10 [6之间变化],2.1 \ ud7 10 [4]和1.1 \ ud7 10 [5]和9.3 \ ud7 10 [1]和1.1 \ ud7 10 [4] CFU g-1。相反,SP沉积物中的氨氧化细菌和硝化细菌的数量低于SCP和MCP沉积物中的细菌。 16S rDNA基因的变性梯度凝胶电泳(DGGE)和树状图分析表明,混合培养环境中细菌的多样性高于单培养环境。 EC与SCP或MCP之间细菌群落的相似性高于EC与SP之间。这些结果表明,海参培养物在影响主要由黄杆菌科(64.3%),拟杆菌(21%)和三角洲变形杆菌(14.7%)组成的环境细菌群落中起着重要作用,包括Croceimarina,Lutibacter,Psychroserpens的属。等等。结果说明了在微生物生态学水平上海虾养殖对虾池塘的好处。

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