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Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX

机译:海洋铁肥实验LOHAFEX中纳米浮游植物群落的稳定组成

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摘要

The iron fertilization experiment LOHAFEX was conducted in a cold-core eddy in the Southern Atlantic Ocean during austral summer. Within a few days after fertilization, a phytoplankton bloom developed dominated by nano- and picoplankton groups. Unlike previously reported for other iron fertilization experiments, a diatom bloom was prevented by iron and silicate co-limitation. We used 18S rRNA gene tag pyrosequencing to investigate the diversity of these morphologically similar cell types within the nano- and picoplankton and microscopically enumerated dominant clades after catalyzed reported deposition fluorescence in situ hybridization (CARD-FISH) with specific oligonucleotide probes. In addition to Phaeocystis, members of Syndiniales group II, clade 10–11, and the Micromonas clades ABC and E made up a major fraction of the tag sequences of the nano- and picoplankton community within the fertilized patch. However, the same clades were also dominant before the bloom and outside the fertilized patch. Furthermore, only little changes in diversity could be observed over the course of the experiment. These results were corroborated by CARD-FISH analysis which confirmed the presence of a stable nano- and picoplankton community dominated by Phaeocystis and Micromonas during the entire course of the experiment. Interestingly, although Syndiniales dominated the tag sequences, they could hardly be detected by CARD-FISH, possibly due to the intracellular parasitic life style of this clade. The remarkable stability of the nano- and picoplankton community points to a tight coupling of the different trophic levels within the microbial food web during LOHAFEX.
机译:在夏季,在南部大西洋南部的一个冷核涡流中进行了铁肥实验LOHAFEX。在受精后的几天内,浮游植物的水华出现,被纳米浮游生物和微型浮游生物所支配。与先前报道的其他铁肥实验不同,铁和硅酸盐的共限制作用可防止硅藻大量溢出。我们使用18S rRNA基因标签焦磷酸测序技术研究了特定形态的寡核苷酸探针催化的沉积荧光原位杂交(CARD-FISH)后,在纳米浮游生物和皮克浮游生物以及微观枚举的优势进化枝中这些形态相似的细胞类型的多样性。除了囊藻外,Syndiniales组II的成员,进化枝10-11,以及Micromonas进化枝ABC和E,在受精斑块中构成了纳米和微微浮游生物群落标签序列的主要部分。然而,同样的进化枝在开花前和受精斑块之外也占主导地位。此外,在实验过程中,仅观察到多样性几乎没有变化。 CARD-FISH分析证实了这些结果,该分析证实了在整个实验过程中,稳定的纳米和微微浮游生物群落的存在,以Phaeocystis和Micromonas为主。有趣的是,尽管Syndiniales主导了标签序列,但由于该进化枝的细胞内寄生生活方式,它们几乎无法被CARD-FISH检测到。在LOHAFEX期间,纳米和微型浮游生物群落的显着稳定性表明微生物食物网中不同营养水平的紧密联系。

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