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Divergent genetic selection for residual feed intake impacts mitochondria reactive oxygen species production in pigs

机译:剩余饲料摄入的不同遗传选择影响猪线粒体活性氧的产生

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摘要

The objective of this study was to determine the extent to which genetic selection for residual feed intake (RFI) impacts electron leakage and reactive oxygen species (ROS) production in mitochondria from muscle and liver tissue. Understanding how genetic selection for RFI impacts animal physiology and growth efficiency is of the utmost importance as the world population increases. Production efficiency is tied directly to energy use. Mitochondria were used in this study because they produce 90% of the ATP in the body and use a large majority of dietary energy. Mitochondria were isolated from both muscle and liver tissue from pigs genetically selected for RFI (n = 8 per RFI line; 34 ± 4 kg). A 2,7-dichlorofluorscein diacetate assay was used to detect differences in hydrogen peroxide production between the more efficient low RFI line and the less efficient high RFI line. Our hypothesis was that greater efficiency would be linked to less ROS production from the mitochondria. There was less ROS production in mitochondria from the white portion of the semitendinosus in the low RFI line compared with the high RFI line, when both NADH and Flavin Adenine Dinucleotide (FADH2) energy substrates were used (glutamate and succinate, respectively). Additionally, mitochondria from the red portion of the semitendinosus in the low RFI line had less ROS production when succinate was used as an energy substrate (P u3c 0.05). A positive correlation was observed between RFI and ROS in mitochondria from the LM. These data indicate genetic selection for RFI may influence mitochondrial ROS production and efficiency of pork production.
机译:这项研究的目的是确定残留饲料摄入量(RFI)的遗传选择影响肌肉和肝组织线粒体中电子泄漏和活性氧(ROS)产生的程度。随着世界人口的增加,了解用于RFI的基因选择如何影响动物生理和生长效率至关重要。生产效率与能源使用直接相关。在这项研究中使用了线粒体,因为它们在体内产生了90%的ATP,并消耗了大部分的饮食能量。从为RFI基因选择的猪的肌肉和肝脏组织中分离出线粒体(每根RFI品系n = 8; 34±4 kg)。 2,7-二氯荧光素二乙酸酯测定法用于检测效率较高的低RFI管线和效率较低的高RFI管线之间过氧化氢生成的差异。我们的假设是更高的效率将与线粒体产生的ROS减少相关。当同时使用NADH和黄素腺嘌呤二核苷酸(FADH2)能量底物(分别为谷氨酸和琥珀酸)时,低RFI谱系中半腱肌白色部分的线粒体中的ROS生成量低于高RFI谱系。此外,当将琥珀酸酯用作能量底物时,来自低RFI谱系中半腱肌红色部分的线粒体具有较少的ROS生成(P <0.05)。 LM线粒体的RFI和ROS之间存在正相关。这些数据表明,RFI的遗传选择可能会影响线粒体ROS的产生和猪肉的生产效率。

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