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Development of a biotransformation process of hydroperoxides into green leaf volatiles using sugar beet leaves

机译:利用甜菜叶将氢过氧化物生物转化为绿色挥发物的方法的开发

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摘要

Natural green leaf volatiles (GLVs) are commonly sole AS aldehydic and alcoholic flavors; their synthesis is a great challenge for industry. Especially, the bioconversion step of fatty acid hydroperoxides into aldehydes by the hydroperoxide lyase (HL). This widely studied enzyme is present in cell membranes of green organs from superior plants. Extracted from its natural condition, HL is subject to a suicidal behavior, being irreversibly inhibited by its own substrate. Furthermore, GLVs produced are highly volatile and quickly degraded by other plant enzymes. Thence, high GLVs levels in industrial production are very difficult to obtain, but several biotechnological tools could be developed to enhance this natural synthesis level more than hundred times. This paper will describe a new method for GLVs production in bioreactor using sugar beet leaves as source of HL. One step reaction, including hydroperoxide metabolisation and GLVs extraction, is performed during a short time process. Downstream processing to dispose of natural and pure GLVs molecule will also be discussed.
机译:天然绿叶挥发物(GLV)通常是AS唯一的醛和酒精香精。它们的合成对工业来说是巨大的挑战。特别地,通过氢过氧化物裂解酶(HL)将脂肪酸氢过氧化物生物转化为醛的步骤。这种被广泛研究的酶存在于优质植物的绿色器官细胞膜中。 HL是从其自然条件中提取的,具有自杀行为,被其自身的底物不可逆地抑制。此外,产生的GLV极易挥发,并被其他植物酶迅速降解。因此,很难在工业生产中获得高GLVs水平,但是可以开发出多种生物技术工具来将这种自然合成水平提高一百倍以上。本文将描述一种以甜菜叶为HL来源的生物反应器中生产GLV的新方法。一步反应,包括氢过氧化物代谢和GLV提取,在短时间内进行。还将讨论处理天然和纯GLV分子的下游工艺。

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