Comparison of pour upon method and wet-dabbing method to judge the disinfecting qualities of building materials

摘要

To evaluate the possibilites of use of building materials in the veterinary and quarantine area of a zoo, twelve materials have been tested for their cleaning and desinfecting qualities. The materials were contaminated with a specified amount of Serratia marcescens germs and afterwards cleaned with cold water only, then with a sponge, warm water and cleansing agent and, finally, disinfected with disinfecting agent. The wet-dabbing method and the Direct Surface Agar Planting-method (DSAP-method) were used in comparison to determine the surface germ count.The main experiment was divided in four parts.First, the recovery rates of the DSAP and wet-dabbing methods were determined for the different materials.In a second experiment, the surfaces were cleaned under running water. Thirdly, the surfaces were cleaned with a sponge, cleansing agent and warm water. And last, the surfaces were disinfected with Lysovet PA. After cleaning and disinfecting the amount of Serratia marcescens germs was determined. With the help of the germ count the building materials were judged for the use in the veteriny and quarantine area.