Screening method for selecting the potential fungi for use in the bioremediation of leachate

摘要

This study focuses on a screening method for selecting the potential fungi which has ability to be used in bioremediation of leachate. In this study, twelve fungal species were tested for their ability to grow on 50% and 100% leachate incorporated with malt extract agar (MEA) and also the affect of pH medium on fungi growth. The radial growth was measured every 48 h for a period of 30 days afterudincubation at 30 °C, and the growth rates were determined.udResults show no growth was observed by all fungi species onudmalt extract agar incorporated with 100% leachate except forudGanoderma australe, Trametes menziesii (KUM 7011(54)),udPenicillium sp., and Pycnoporus sanguineus. These fungi giveudgrowth rates of 6.1 ± 0.8 mm, 5.9 ± 0.6 mm, 2.3 ± 0.3 mm andud0.8 ± 0.3 mm respectively. On 50% leachate, the growth ofudTrametes menziesii shows the most rapid followed byudGanoderma australe and Penicillium sp. with growth rates ofud7.4 ± 0.3, mm 6.9 ± 0.3 mm, and 2.9 ± 0.0 mm respectively.udBesides, the comparison growth of Penicillium sp., Trametesudmenziesii, and Ganoderma australe on the medium at pH 6.0 –ud6.5 and nonadjusted pH medium show slightly difference. Theyudgive the growth rates of 3.7 ± 0 and 2.9 ± 0, 7.0 ± 0.1 and 7.4 ± 0.3, 6.1 ± 0.3 and 6.4 ± 0.1 respectively. Therefore, these fungi species show the ability to grow on leachate and can be considered to have the potential to be used in theudbioremediation of leachate.