DEGRADATION OF PAHs BY SOIL FUNGI WITH POTENTIAL FOR BIOREMEDIATION

摘要

The five fungal strains used is this work, namely 710, 870, 898,984 and 1010, were a gift from Funda??o Tropical André Toselo, and were isolated from soil samples collected at the Jureia-Itatins Ecological Reserve. Following microscopically examinations these strains were classified as Acremoniun sp. (710), Trichoderma sp. (870), Fusarium sp. (898), Aspergillus sp. (984) and Verticillium sp. (1040). The following Polycyclic Aromatic Hydrocarbons (PAHs): naphthalene (0,5%), anthracene (0,1%), pyrene (0,05%) and benzo[a]pyrene (0,05%), were used throughout this work, as the carbon source. The strains were then cultivated in the PAHs, added on the 3rd day of growth, and incubated for a total period of 33 days, with samples consisting of duplicates of Erlenmeyer flasks collected on days 3, 6, 9, 12, 15, 20, 25 and 30. The presence of ligninolytic enzymes, biosurfactants, cytochrome P-450 (cit P450) and the degradation of the PAHS were determined. The results showed that the strains were able to grow and degrade the PAHs, as complex as pyrene and benzo[a]pyrene, and the percentage of degradation, the production of ligninolytic enzymes, cit P-450 and biosurfactants, varied with the strain, the carbon source and with the day of growth. Manganese-peroxidase was the predominant enzyme produced by the five fungi.