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Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins

机译:绘制负责拟南芥MADS域器官同一性蛋白质功能特异性的蛋白质区域

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摘要

The Arabidopsis MADS domain proteins AP1, AP3, PI, and AG specify floral organ identity. All of these proteins contain a MADS domain required for DNA binding and dimerization; a region termed L (linker between MADS domain and K domain), which plays an important role in dimerization specificity; the K domain, named for its similarity to the coiled-coil domain of keratin; and a C-terminal region of unknown function. To determine which regions of these proteins are responsible for their abilities to specify different organs, we have made a number of chimeric MADS box genes. The in vivo function of these chimeric genes was investigated by ectopic expression in transgenic Arabidopsis plants. The four proteins fall into two classes on the basis of regions responsible for their functional specificities. The L region and K domain define the functional specificities of AP3 and PI, while the MADS domain and L region define the functional specificities of AP1 and AG.
机译:拟南芥MADS结构域蛋白AP1,AP3,PI和AG指定花器官身份。所有这些蛋白质都包含DNA结合和二聚化所需的MADS结构域。称为L(MADS结构域和K结构域之间的连接子)的区域,在二聚化特异性中起重要作用; K结构域,由于其与角蛋白的卷曲螺旋结构域的相似性而命名;和未知功能的C端区域。为了确定这些蛋白质的哪些区域负责它们指定不同器官的能力,我们制备了许多嵌合的MADS box基因。通过在转基因拟南芥植物中的异位表达研究了这些嵌合基因的体内功能。根据负责其功能特异性的区域,这四种蛋白质分为两类。 L区和K区定义了AP3和PI的功能特异性,而MADS区和L区定义了AP1和AG的功能特异性。

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