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Interfacial Reactions of Ozone with Surfactant Protein B in a Model Lung Surfactant System

机译:在模型肺表面活性剂系统中,臭氧与表面活性蛋白B的界面反应

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摘要

Oxidative stresses from irritants such as hydrogen peroxide and ozone (O_3) can cause dysfunction of the pulmonary surfactant (PS) layer in the human lung, resulting in chronic diseases of the respiratory tract. For identification of structural changes of pulmonary surfactant protein B (SP-B) due to the heterogeneous reaction with O_3, field-induced droplet ionization (FIDI) mass spectrometry has been utilized. FIDI is a soft ionization method in which ions are extracted from the surface of microliter-volume droplets. We report structurally specific oxidative changes of SP-B_(1−25) (a shortened version of human SP-B) at the air−liquid interface. We also present studies of the interfacial oxidation of SP-B_(1−25) in a nonionizable 1-palmitoyl-2-oleoyl-sn-glycerol (POG) surfactant layer as a model PS system, where competitive oxidation of the two components is observed. Our results indicate that the heterogeneous reaction of SP-B_(1−25) at the interface is quite different from that in the solution phase. In comparison with the nearly complete homogeneous oxidation of SP-B_(1−25), only a subset of the amino acids known to react with ozone are oxidized by direct ozonolysis in the hydrophobic interfacial environment, both with and without the lipid surfactant layer. Combining these experimental observations with the results of molecular dynamics simulations provides an improved understanding of the interfacial structure and chemistry of a model lung surfactant system subjected to oxidative stress.
机译:来自刺激物(如过氧化氢和臭氧(O_3))的氧化应激可导致人肺中的肺表面活性剂(PS)层功能障碍,从而导致呼吸道慢性疾病。为了鉴定由于与O_3的异质反应而导致的肺表面活性剂蛋白B(SP-B)的结构变化,已使用了场致液滴电离(FIDI)质谱。 FIDI是一种软电离方法,其中从微升体积的液滴表面提取离子。我们报告了气液界面处SP-B_(1-25)(人SP-B的简化版本)的结构特异性氧化变化。我们还作为模型PS系统介绍了在不可电离的1-棕榈酰基-2-油酰基-sn-甘油(POG)表面活性剂层中SP-B_(1-25)的界面氧化的研究,其中两种组分的竞争性氧化是观测到的。我们的结果表明,界面处SP-B_(1-25)的异质反应与溶液相的异质反应有很大不同。与SP-B_(1-25)的几乎完全均相氧化相比,已知和臭氧发生反应的氨基酸只有一部分在有和没有脂质表面活性剂层的疏水性界面环境中通过直接臭氧分解而被氧化。将这些实验观察结果与分子动力学模拟的结果结合起来,可以更好地理解遭受氧化应激的模型肺表面活性剂系统的界面结构和化学性质。

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