首页> 外文OA文献 >Phenazines and Other Redox-Active Antibiotics Promote Microbial Mineral Reduction
【2h】

Phenazines and Other Redox-Active Antibiotics Promote Microbial Mineral Reduction

机译:吩嗪和其他具有氧化还原活性的抗生素促进微生物矿物质的减少

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Natural products with important therapeutic properties are known to be produced by a variety of soil bacteria, yet the ecological function of these compounds is not well understood. Here we show that phenazines and other redox-active antibiotics can promote microbial mineral reduction. Pseudomonas chlororaphis PCL1391, a root isolate that produces phenazine-1-carboxamide (PCN), is able to reductively dissolve poorly crystalline iron and manganese oxides, whereas a strain carrying a mutation in one of the phenazine-biosynthetic genes (phzB) is not; the addition of purified PCN restores this ability to the mutant strain. The small amount of PCN produced relative to the large amount of ferric iron reduced in cultures of P. chlororaphis implies that PCN is recycled multiple times; moreover, poorly crystalline iron (hydr)oxide can be reduced abiotically by reduced PCN. This ability suggests that PCN functions as an electron shuttle rather than an iron chelator, a finding that is consistent with the observation that dissolved ferric iron is undetectable in culture fluids. Multiple phenazines and the glycopeptidic antibiotic bleomycin can also stimulate mineral reduction by the dissimilatory iron-reducing bacterium Shewanella oneidensis MR1. Because diverse bacterial strains that cannot grow on iron can reduce phenazines, and because thermodynamic calculations suggest that phenazines have lower redox potentials than those of poorly crystalline iron (hydr)oxides in a range of relevant environmental pH (5 to 9), we suggest that natural products like phenazines may promote microbial mineral reduction in the environment.
机译:具有重要治疗特性的天然产物已知是由多种土壤细菌产生的,但是这些化合物的生态功能尚未得到很好的了解。在这里,我们表明吩嗪和其他氧化还原活性抗生素可以促进微生物矿物质的减少。绿假单胞菌PCL1391是产生吩嗪-1-羧酰胺(PCN)的根系分离物,能够还原溶解结晶性较弱的铁和锰氧化物,而在吩嗪生物合成基因之一(phzB)中携带突变的菌株则不能。加入纯化的PCN可以使突变菌株恢复这种能力。相对于在绿腐病菌培养物中还原的大量三价铁,生成的PCN量较少,这意味着PCN可以循环使用多次。此外,通过减少PCN可以非结晶性地还原​​结晶性较弱的氢氧化铁。这种能力表明PCN充当电子梭而不是铁螯合剂,这一发现与在培养液中无法检测到溶解的三价铁的观察结果一致。多种吩嗪和糖肽抗生素博来霉素还可以通过异化铁还原细菌希瓦氏菌(Shewanella oneidensis)MR1刺激矿物质还原。由于无法在铁上生长的多种细菌菌株可以还原吩嗪,并且由于热力学计算表明,在相关的环境pH值(5至9)范围内,吩嗪的氧化还原电势低于结晶性较弱的铁(氢氧化物)的氧化还原电势。吩嗪等天然产物可能会促进环境中微生物矿物质的减少。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号