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Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications

机译:花卉调节剂FLC和SOC1通过拮抗染色质修饰直接调节FLC和SVP 1的B3型转录因子靶标的B3型转录因子靶标在拟南芥中的表达

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摘要

Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition.
机译:植物射击公司环境和内源性线索的整合决定了开花和生殖发展的时机。拟南芥的疯子箱转录因子开花基因座C(FLC)是一种重要的花卉过渡的重要阻遏物,其在植物暴露在冬季感冒之前阻断开花。然而,FLC的靶基因尚未详细描述,我们对FLC抑制这些目标的转录的机制以及如何在花卉过渡期间克服这种抑制的机制仍然是零碎的。这里,我们鉴定并表征FLC和SVP1(TFS1)的靶标,FLC的新靶基因及其相互作用的蛋白质短营养相(SVP)。 TFS1编码B3型转录因子,我们表明TFS1突变体稍后比野生型,特别是在短期内开花。 FLC和SVP抑制TFS1转录,导致组蛋白3(H3K27ME3)的肝脏27的三甲基化沉积在TFS1基因座处的Polycomb抑制复合物2。在花卉过渡期间,通过冷FLC下调,TFS1转录被Conscrans1(SoC1)过表达的抑制剂,由FLC / SVP的另一种靶标编码的MADS箱蛋白促进。 SOC1通过募集早期开花6(REF6)和SWI / SNF染色质Remodeler ATPase Brahma(BRM)的组蛋白脱甲基化酶相对于TFS1的PRC功能。在TFS1在TFS1在TFS1结合到通过介体络合物,还严格需要对BRM的募集的募集性促进BRM的募集性偶联蛋白质样9(SPL9)。因此,我们表明,由不同浊箱转录因子复合物介导的拮抗染色质修饰在花卉过渡期间定义FLC / SVP下游的相互作用网络内基因的时间和空间模式以及在FLC / SVP的相互作用网络中进行的时间和空间模式起着至关重要的作用。

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