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Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population

机译:匈牙利荷斯坦-弗里斯兰公牛大坝种群生长激素基因的AluI多态性与生产和繁殖性状的关联

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摘要

The aim of this paper was to study the polymorphisms of bovine growth hormone gene. The authors genotyped363 Hungarian Holstein-Friesian bull dams from 6 farms all over the country. Two variants (L and V) of thebovine growth hormone gene digested with AluI enzyme were identified in the experiment. Genotyping wascarried out by PCR-RFLP method.The frequency data of L and V allele was 0.93 and 0.07 respectively. Distribution of the three genotypes were87.05% (LL), 12.40% (LV) and 0.55% (VV). The studied population was in H-W equilibrium considering thegenotype distribution.SPSS 11.0 for Windows was used to reveal the possible correlations between GH genotypes and production andreproduction traits and further statistical analyses.On the basis of statistical analyses it can be found that VV genotype cows had the longest milking period and LLhad the shortest dry period. Both differences were significant. Cows with LV genotype had significantly highertest milking data than LL cows. Furthermore, LV genotype seemed to be advantageous for 305 days lactationmilk yield. While milk composition traits, as 305 days milk fat and protein percent showed the oppositetendency, since LL genotyped dams produced significantly higher values in these traits.
机译:本文的目的是研究牛生长激素基因的多态性。作者对来自全国6个农场的363个匈牙利荷斯坦-弗里斯兰公牛水坝进行了基因分型。在实验中鉴定了用AluI酶消化的牛生长激素基因的两个变体(L和V)。采用PCR-RFLP方法进行基因分型,L和V等位基因频率数据分别为0.93和0.07。三种基因型的分布分别为87.05%(LL),12.40%(LV)和0.55%(VV)。考虑到基因型分布,研究的种群处于HW平衡。使用Windows的SPSS 11.0揭示GH基因型与生产和繁殖性状之间可能的相关性,并进行进一步的统计分析。在统计分析的基础上,可以发现VV基因型奶牛具有挤奶时间最长,LL的干燥时间最短。两种差异均显着。 LV基因型奶牛的泌乳数据明显高于LL奶牛。此外,LV基因型似乎对305天的泌乳产奶量有利。牛奶组成特征在305天时显示出相反的趋势,因为LL基因型大坝在这些特征上产生了更高的价值。

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