中国荷斯坦奶牛FoxO1基因多态性及与繁殖性状关系研究

摘要

PCR-single-strand conformation polymorphism (PCR-SSCP) and sequencing are used and a novel single nucleotide polymorphisms (SNP)was detected in the forth exon of FoxO1 gene.The gene types of the 925 Holsteins were differentiated by PCR-restriction fragment length polymorphism (PCR-RFLP).The result showed that there were three genotypes (CC,CT,TT) and the frequency of allele C which was superior gene was 0.95; x2 fitness test showed that the loci deviates from the Hardy-Weinberg equilibrium (P＜0.05).The correlation analysis revealed that these polymorphisms had a extremely significant effects on calving interval,open days and the first calving age (P＜0.05),but there was not a significant difference with the days between calving and oestrus,the times of service per conception and birth weight (P＞0.05).%通过PCR-单链构象多态性(PCR-SSCP)结合测序技术发现FoxO1第4外显子一个新单核苷酸多态性(SNP),限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术分别分析925头中国荷斯坦奶牛FoxO1该位点多态性.结果表明,在中国荷斯坦奶牛中,FoxO1基因有3个基因型,分别是CC、TT、CT.其中等位基因C频率为0.95,是优势等位基因;x2适合性检验表明该位点不符合哈代温伯格平衡(P,＜0.05).该基因不同基因型对奶牛产犊间隔、空怀期以及初产日龄有显著影响(P＜0.05),而对产犊到发情、犊牛初生重、配种次数影响不显著(P＞0.05).