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骨欠損再建における培養細胞シートを併用した培養人工骨移植の有用性

机译:人工骨移植联合培养细胞片修复骨缺损的有效性

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摘要

AIM: To determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate (TCP) constructs on bone formation in bone defects.METHODS: Osteogenic matrix cell sheets were prepared from bone marrow stromal cells (BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds (1) seeded with BMSCs; (2) wrapped with osteogenic matrix cell sheets; or (3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk.RESULTS: In bone defects, constructs implanted with cell sheets showed callus formation with segmental or continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone non-union. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness (mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP constructs.CONCLUSION: This technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects.
机译:目的:确定成骨基质细胞片和β-磷酸三钙(TCP)移植物对骨缺损骨形成的影响。方法:由骨髓基质细胞(BMSCs)和多孔TCP陶瓷制备成骨基质细胞片被用作脚手架。制备了三个实验组,包括TCP支架(1),其接种有BMSC。 (2)包裹成骨基质细胞片;或(3)两者。将构建体植入大鼠股骨缺损模型中,并在8周后通过射线照相,组织学,生物化学和力学测试评估骨生长。结果:在骨缺损中,植入细胞片的构建体显示愈伤组织形成,并在该处形成节段性或连续性骨形成。与使用BMSC播种的TCP相比,它每周8周,导致骨骼不愈合。与用BMSCs播种的常规骨组织工程TCP支架相比,用成骨基质细胞片包裹TCP构建物可增加其成骨潜能并导致骨形成。具有连续骨形成的BMSC / TCP / Sheet结构,具有分段骨形成的BMSC / TCP / Sheet结构和BMSC / TCP的抗压刚度(平均值±SD)值为225.0±95.7、30.0±11.5和26.3±10.6 MPa构造。具有连续骨形成的BMSC / TCP / Sheet结构的抗压刚度明显高于具有分段骨形成和BMSC / TCP结构的BMSC / TCP / Sheet。结论:该技术是对现有方法(例如TCP替换)的改进,可用于硬组织重建和早期骨缺损的诱导。

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