Aspects of Modelling and Control of Bioprocesses: Application of ConventionalApproach and Functional State Concept

摘要

A mammalian cell culture is a very complex process. With the increasing demand ofmonoclonal antibodies (MAb) in therapy, diagnostic and chemical assays, the study of high density and high production culture of mammalian cells is becoming more and more important. In order to monitor and control the process and possibly increase the production of monoclonal antibodies, a modelling study of the process is needed. An unstructured model (a global model) for Murine myeloma cell culture will be presented for that purpose in this chapter. In Section 2.1, metabolic, dynamic and modelling studies of mammalian cell cultures will be introduced on the basis of the literature. Materials and methods are described in Section 2.2. A global model based on mass balance and metabolic phenomena is used to describe the animal cell growth, and the formation of by-products and monoclonal antibodies (Section 2.3). In Section 2.4 of the chapter, the process is simulated with the developed model, which could be simplified partly by mathematical analysis of the model parameters. Section 2.5 will be discussion and conclusions. (Copyright (c) Helsinki University of Technology 1995.)