CALUX Generation 3 – Enhanced Sensitivity for Low Volume/Low Concentration Samples.

摘要

Dr. Michael Denison’s SRP-funded research at the University of California-Davis SRP led to the development and validation of a series of mechanistically-based bioassays for rapid and inexpensive detection of dioxin-like HAHs. To develop the Chemically Activated Luciferase Expression bioassay (CALUX), the researchers placed a firefly luciferase gene under the control of the AhR and inserted it into a mouse cell line. Dioxin-like HAHs in a sample bind to the AhR and activate the receptor. The AhR-contaminant complex then travels into the nucleus of the cell and binds to specific sequences in the DNA (dioxin responsive elements [DREs]). This induces expression of firefly luciferase, which results in dose-dependent production of light that is easily measured and provides accurate and reproducible detection and relative quantitation of dioxin-like chemicals in a wide variety of biological, environmental, and food/feed samples.